PMID- 18498620
OWN - NLM
STAT- MEDLINE
DCOM- 20090318
LR  - 20220330
IS  - 1471-2172 (Electronic)
IS  - 1471-2172 (Linking)
VI  - 9
DP  - 2008 May 22
TI  - The role of Toll-like receptor-4 in pertussis vaccine-induced immunity.
PG  - 21
LID - 10.1186/1471-2172-9-21 [doi]
AB  - BACKGROUND: The gram-negative bacterium Bordetella pertussis is an important 
      causative agent of pertussis, an infectious disease of the respiratory tract. 
      After introduction of whole-cell vaccines (wP) in the 1950's, pertussis incidence 
      has decreased significantly. Because wP were found to be reactogenic, in most 
      developed countries they have been replaced by acellular vaccines (aP). We have 
      previously shown a role for Toll-like receptor 4 (Tlr4) in pertussis-infected 
      mice and the pertussis toxin (Ptx)-IgG response in wP-vaccinated children, 
      raising the issue of the relative importance of Tlr4 in wP vaccination of mice. 
      Here we analyze the effects of wP and aP vaccination and B. pertussis challenge, 
      in Tlr4-deficient C3H/HeJ and wild-type C3H/HeOuJ mice. aP consists of Ptx, 
      filamentous hemagglutinin (FHA), and pertactin (Prn). RESULTS: We show an 
      important role of Tlr4 in wP and (to a lesser extent) aP vaccination, induction 
      of Th1 and Th17 cells by wP but not aP vaccination, and induction of Th17 cells 
      by infection, confirming data by Higgins et al. (J Immunol 2006, 177:7980-9). 
      Furthermore, in Tlr4-deficient mice, compared to wild-type controls (i) after 
      vaccination only, Ptx-IgG (that was induced by aP but not wP vaccination), 
      FHA-IgG, and Prn-IgG levels were similar, (ii) after infection (only), lung 
      IL-1alpha and IL-1beta expression were lower, (iii) after wP vaccination and 
      challenge, Prn-IgG level and lung IL-5 expression were higher, while lung 
      IL-1beta, TNF-alpha, IFN-gamma, IL-17, and IL-23 expression were lower, and lung 
      pathology was absent, and (iv) after aP vaccination and challenge, Prn-IgG level 
      and lung IL-5 expression were higher, while Ptx-IgG level was lower. CONCLUSION: 
      Tlr4 does not influence the humoral response to vaccination (without challenge), 
      plays an important role in natural immunity, wP and aP efficacy, and induction of 
      Th1 and Th17 responses, is critical for lung pathology and enhances 
      pro-inflammatory cytokine production after wP vaccination and challenge, and 
      diminishes Th2 responses after both wP and aP vaccination and challenge. wP 
      vaccination does not induce Ptx-IgG. A role for LPS in the efficacy of wP 
      underlines the usefulness of LPS analogs to improve bacterial subunit vaccines 
      such as aP.
FAU - Banus, Sander
AU  - Banus S
AD  - Health Protection Research, National Institute of Public Health and the 
      Environment, 3720 BA Bilthoven, The Netherlands. sander.banus@rivm.nl
FAU - Stenger, Rachel M
AU  - Stenger RM
FAU - Gremmer, Eric R
AU  - Gremmer ER
FAU - Dormans, Jan A M A
AU  - Dormans JA
FAU - Mooi, Frits R
AU  - Mooi FR
FAU - Kimman, Tjeerd G
AU  - Kimman TG
FAU - Vandebriel, Rob J
AU  - Vandebriel RJ
LA  - eng
PT  - Journal Article
DEP - 20080522
PL  - England
TA  - BMC Immunol
JT  - BMC immunology
JID - 100966980
RN  - 0 (Cytokines)
RN  - 0 (Pertussis Vaccine)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Vaccines, Acellular)
SB  - IM
MH  - Animals
MH  - Bordetella pertussis/*immunology
MH  - Cytokines/metabolism
MH  - Immunity, Active
MH  - Lymphocyte Activation/drug effects/immunology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Knockout
MH  - Pertussis Vaccine/*immunology/therapeutic use
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
MH  - Toll-Like Receptor 4/*immunology/*metabolism
MH  - Vaccination
MH  - Vaccines, Acellular/*immunology/therapeutic use
MH  - Whooping Cough/*immunology/pathology/*prevention & control
PMC - PMC2409298
EDAT- 2008/05/24 09:00
MHDA- 2009/03/19 09:00
PMCR- 2008/05/22
CRDT- 2008/05/24 09:00
PHST- 2007/09/29 00:00 [received]
PHST- 2008/05/22 00:00 [accepted]
PHST- 2008/05/24 09:00 [pubmed]
PHST- 2009/03/19 09:00 [medline]
PHST- 2008/05/24 09:00 [entrez]
PHST- 2008/05/22 00:00 [pmc-release]
AID - 1471-2172-9-21 [pii]
AID - 10.1186/1471-2172-9-21 [doi]
PST - epublish
SO  - BMC Immunol. 2008 May 22;9:21. doi: 10.1186/1471-2172-9-21.