PMID- 18500740
OWN - NLM
STAT- MEDLINE
DCOM- 20081027
LR  - 20091211
IS  - 1552-4957 (Electronic)
IS  - 1552-4949 (Linking)
VI  - 74
IP  - 5
DP  - 2008 Sep
TI  - Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis.
PG  - 282-6
LID - 10.1002/cyto.b.20426 [doi]
AB  - BACKGROUND: Marginal zone B-cell lymphoma (MZL) comprises three related yet 
      biologically distinct subtypes--splenic MZL (SMZL), nodal MZL (NMZL), and 
      extranodal MZL of MALT type (MALT). In cases without adequate morphology, 
      immunophenotypic characterization by flow cytometric immunophenotyping (FCI) 
      relies heavily on exclusion of other low-grade lymphomas. We performed a 
      retrospective review of FCI studies of MZL to search for immunophenotypes 
      specific for MZL and its subtypes. We compared these to follicular lymphoma (FL) 
      as we were specifically interested in differentiating MZL from CD10 negative FL. 
      DESIGN: FCI findings for MZL and FL cases were reviewed. Statistical analysis of 
      patterns and intensity of antigen expression [mean channel fluorescence (MCF)] 
      were performed. RESULTS: Thirty-one cases of MZL (7 SMZL, 6 NMZL, 15 MALT, 3 MZL 
      not otherwise specified) and 31 cases of FL were identified. All expressed CD19, 
      CD20, and CD45. Thirty-two percent of MZL and 77% of FL expressed CD38. 
      Expression of CD11c was seen in 57% of SMZL and 8% of other MZL (P < 0.01). 
      Statistically significant differences in antigen expression between MZL and FL 
      were seen for CD10, CD11c, and CD38. CD19 expression was significantly brighter 
      in MZL (mean MCF of 455.3) than in FL (mean MCF of 166.9) (P < 0.001). MCF for 
      isotype controls and CD20 were similar for FL and MZL. CONCLUSIONS: MZL expresses 
      typical pan-B-cell antigens. Expression of CD11c is highly associated with SMZL. 
      Levels of CD19 expression in conjunction with CD11c and CD38 expression can 
      distinguish MZL from CD10 negative FL.
FAU - Kost, Christine B
AU  - Kost CB
AD  - Department of Pathology and Laboratory Medicine, Emory University School of 
      Medicine, Atlanta, GA 30322, USA.
FAU - Holden, Jeannine T
AU  - Holden JT
FAU - Mann, Karen P
AU  - Mann KP
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
RN  - 0 (Antibodies, Neoplasm)
RN  - 0 (Antigens, Neoplasm)
SB  - IM
MH  - Antibodies, Neoplasm/immunology
MH  - Antigens, Neoplasm/immunology
MH  - Fluorescence
MH  - Humans
MH  - Immunophenotyping/*methods
MH  - Lymphoma, B-Cell, Marginal Zone/*diagnosis/immunology
MH  - Lymphoma, Follicular/immunology
MH  - Retrospective Studies
EDAT- 2008/05/27 09:00
MHDA- 2008/10/28 09:00
CRDT- 2008/05/27 09:00
PHST- 2008/05/27 09:00 [pubmed]
PHST- 2008/10/28 09:00 [medline]
PHST- 2008/05/27 09:00 [entrez]
AID - 10.1002/cyto.b.20426 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2008 Sep;74(5):282-6. doi: 10.1002/cyto.b.20426.