PMID- 18501512
OWN - NLM
STAT- MEDLINE
DCOM- 20080924
LR  - 20141120
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 439
IP  - 1
DP  - 2008 Jul 4
TI  - Stimulatory effects of a melatonin receptor agonist, ramelteon, on BDNF in mouse 
      cerebellar granule cells.
PG  - 34-6
LID - 10.1016/j.neulet.2008.04.099 [doi]
AB  - Melatonin receptor activation has been linked to the regulation of neurotrophic 
      factors, including the brain-derived neurotrophic factor (BDNF). To further 
      characterize the effects of melatonin receptor stimulation on neuronal BDNF, we 
      used a clinically available novel agonist for MT1 and MT2 melatonin receptors, 
      ramelteon. Primary cultures of cerebellar granule cells (CGC) have been 
      established as an in vitro model for studying neuronal BDNF. We took advantage of 
      the availability of MT1- and MT2-deficient (knockout; KO) mice to employ primary 
      CGC prepared from wild type (WT), MT1 KO, and MT2 KO mice. We investigated the 
      effects of ramelteon on BDNF protein and mRNA content. Administered in a low 
      nanomolar range, ramelteon increased BDNF protein content in all three types of 
      mouse CGC. This ramelteon-triggered BDNF protein elevation was not preceded by a 
      BDNF mRNA increase. However, it was prevented by treatment of cultures with a 
      protein synthesis inhibitor cycloheximide. These results demonstrated that the 
      MT1/MT2 melatonin receptor agonist ramelteon is capable of increasing BDNF 
      protein in neurons expressing either of the two melatonin receptor types and that 
      this action of ramelteon involves translational mechanisms. Further research is 
      needed to explore the putative influence of ramelteon on BDNF-associated 
      neuroplasticity.
FAU - Imbesi, Marta
AU  - Imbesi M
AD  - The Psychiatric Institute, Department of Psychiatry, University of Illinois at 
      Chicago, 1601 West Taylor Street, MC912 Chicago, IL 60612, USA.
FAU - Uz, Tolga
AU  - Uz T
FAU - Dzitoyeva, Svetlana
AU  - Dzitoyeva S
FAU - Manev, Hari
AU  - Manev H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080502
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Indenes)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Melatonin, MT1)
RN  - 0 (Receptor, Melatonin, MT2)
RN  - 901AS54I69 (ramelteon)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/classification/genetics/*metabolism
MH  - Cells, Cultured
MH  - Cerebellum/*cytology
MH  - Cycloheximide/pharmacology
MH  - Drug Interactions
MH  - Gene Expression Regulation/*drug effects/genetics
MH  - Indenes/*pharmacology
MH  - Mice
MH  - Mice, Knockout
MH  - Neurons/*drug effects
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA, Messenger/metabolism
MH  - Receptor, Melatonin, MT1/deficiency
MH  - Receptor, Melatonin, MT2/deficiency
MH  - Time Factors
EDAT- 2008/05/27 09:00
MHDA- 2008/09/25 09:00
CRDT- 2008/05/27 09:00
PHST- 2008/03/24 00:00 [received]
PHST- 2008/04/29 00:00 [revised]
PHST- 2008/04/30 00:00 [accepted]
PHST- 2008/05/27 09:00 [pubmed]
PHST- 2008/09/25 09:00 [medline]
PHST- 2008/05/27 09:00 [entrez]
AID - S0304-3940(08)00605-8 [pii]
AID - 10.1016/j.neulet.2008.04.099 [doi]
PST - ppublish
SO  - Neurosci Lett. 2008 Jul 4;439(1):34-6. doi: 10.1016/j.neulet.2008.04.099. Epub 
      2008 May 2.