PMID- 18501920
OWN - NLM
STAT- MEDLINE
DCOM- 20080908
LR  - 20111117
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 336
IP  - 2
DP  - 2008 Jul 31
TI  - Detection and characterization of antibodies against recombinant human 
      erythropoietin by RIPA, ELISA and neutralization assay in patients with renal 
      anemia.
PG  - 152-8
LID - 10.1016/j.jim.2008.04.009 [doi]
AB  - The aim of the present study was to analyze the relations between the serum 
      anti-erythropoietin antibody (AEAb) levels and the antibodies' neutralizing 
      activity in 20 patients with renal anemia and rhEPO-induced antibodies. AEAb 
      levels were determined by the enzyme-linked immunosorbent assay (ELISA, double 
      antigen-bridging) and by radioimmunoprecipitation assay (RIPA). The bone marrow 
      neutralization test was used to determine the neutralizing activity of the 
      antibodies. RIPA and ELISA data resulted in closely correlated measurements. The 
      relations between AEAb levels and the neutralizing activity of the antibodies are 
      variable as shown by follow-up and cross-sectional evaluations of the data. Serum 
      samples with a high antibody level (>1000 ng/ml) are associated with 100% 
      neutralizing activity, whereas serum samples with lower AEAb levels show partial 
      neutralizing activities or have no effect. Determining the neutralizing activity 
      might be helpful when it comes to deciding of whether or not rhEPO therapy should 
      be continued, specifically in patients who have low antibody levels. The apparent 
      affinity of the AEAb as defined by inhibition of the binding of rhEPO (IC(50)) 
      did not change in the course of the disease, nor did it correlate to the AEAb 
      levels or the neutralizing activities.
FAU - Gross, Johann
AU  - Gross J
AD  - Molecular Biology Research Laboratory, Department of Otorhinolaryngology, 
      University Medicine Charite, Berlin, Germany. johann.gross@charite.de
FAU - Moller, Renate
AU  - Moller R
FAU - Bischoff, Steffen
AU  - Bischoff S
FAU - Canaan-Kuhl, Sima
AU  - Canaan-Kuhl S
FAU - Fromme, Manfred
AU  - Fromme M
FAU - Henke, Wolfgang
AU  - Henke W
LA  - eng
PT  - Journal Article
DEP - 20080505
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antibodies)
RN  - 0 (Recombinant Proteins)
RN  - 11096-26-7 (Erythropoietin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Anemia/blood/*immunology
MH  - Antibodies/*blood/immunology
MH  - Antibody Affinity
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Erythropoietin/*immunology
MH  - Female
MH  - Humans
MH  - Kidney Diseases/*immunology
MH  - Male
MH  - Middle Aged
MH  - Neutralization Tests/*methods
MH  - Radioimmunoprecipitation Assay/*methods
MH  - Recombinant Proteins
MH  - Renal Insufficiency/immunology
EDAT- 2008/05/27 09:00
MHDA- 2008/09/09 09:00
CRDT- 2008/05/27 09:00
PHST- 2007/11/22 00:00 [received]
PHST- 2008/04/09 00:00 [revised]
PHST- 2008/04/10 00:00 [accepted]
PHST- 2008/05/27 09:00 [pubmed]
PHST- 2008/09/09 09:00 [medline]
PHST- 2008/05/27 09:00 [entrez]
AID - S0022-1759(08)00140-3 [pii]
AID - 10.1016/j.jim.2008.04.009 [doi]
PST - ppublish
SO  - J Immunol Methods. 2008 Jul 31;336(2):152-8. doi: 10.1016/j.jim.2008.04.009. Epub 
      2008 May 5.