PMID- 18502401
OWN - NLM
STAT- MEDLINE
DCOM- 20080909
LR  - 20131121
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1217
DP  - 2008 Jun 27
TI  - Striatal carotid body graft promotes differentiation of neural progenitor cells 
      into neurons in the olfactory bulb of adult hemiparkisonian rats.
PG  - 213-20
LID - 10.1016/j.brainres.2008.04.015 [doi]
AB  - Progenitor cells generated in the subventricular zone (SVZ) migrate toward the 
      olfactory bulb (OB), where they differentiate into neurons. Growth factors have 
      been shown to promote neurogenesis in the SVZ/OB-system while dopaminergic lesion 
      exerts an opposite effect. As carotid body (CB) cells express growth factors here 
      we study the impact of intrastriatal CB graft on migration and differentiation of 
      neural progenitor cells in the hemiparkinsonian rat SVZ/OB-system. 
      Bromodeoxyuridine (BrdU) was given to intact, 6-hydroxydopamine (6-OHDA)-lesioned 
      and 6-OHDA-lesioned animals transplanted with vehicle or rat CB cells. The 
      migration of progenitor cells was assessed by the quantification of BrdU-labeled 
      cells in the SVZ/OB-system and the neuronal differentiation by the proportion of 
      newborn neurons in the OB. The graft survival was confirmed by CB cell morphology 
      and their tyrosine hydroxylase expression. Some of these CB cells were stained 
      with BrdU, thus indicating their ability for self-renewal. Grafted glomus cells 
      also expressed brain derived neurotrophic factor (BDNF), glial derived 
      neurotrophic factor (GDNF), epidermal growth factor (EGF) and vascular 
      endothelial growth factor (VEGF). The migration of neural progenitor cells was 
      significantly decreased in 6-OHDA-lesioned respect to intact animals. We found a 
      similar number of BrdU-labeled cells in sham-operated than in CB-grafted animals, 
      suggesting that CB graft has no effect on progenitor cell migration. CB-grafted 
      animals exhibited a significantly larger percentage of newborn cells 
      (BrdU/Neuronal Nuclei-labeled cells) respect to 6-OHDA-lesioned and sham-operated 
      animals. This study suggests that striatal CB graft might promote differentiation 
      of SVZ progenitor cells into neurons, probably by the growth factors contained in 
      CB cells.
FAU - Belzunegui, Silvia
AU  - Belzunegui S
AD  - Laboratory of Regenerative Therapy, Department of Neurology, Neuroscience 
      Division, Center for Applied Medical Research, University of Navarra, Avda. Pio 
      XII 55, Pamplona, Spain.
FAU - Izal-Azcarate, Amaya
AU  - Izal-Azcarate A
FAU - San Sebastian, Waldy
AU  - San Sebastian W
FAU - Garrido-Gil, Pablo
AU  - Garrido-Gil P
FAU - Vazquez-Claverie, Marianne
AU  - Vazquez-Claverie M
FAU - Lopez, Berta
AU  - Lopez B
FAU - Marcilla, Irene
AU  - Marcilla I
FAU - Luquin, Ma Rosario
AU  - Luquin MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080416
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Nerve Growth Factors)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Bromodeoxyuridine/metabolism
MH  - Carotid Body/cytology/metabolism/*transplantation
MH  - Cell Differentiation/physiology
MH  - Cell Movement/physiology
MH  - Corpus Striatum/*cytology/metabolism/surgery
MH  - Graft Survival
MH  - Male
MH  - Nerve Growth Factors/biosynthesis
MH  - Neurons/*cytology
MH  - Olfactory Bulb/*cytology
MH  - Parkinsonian Disorders/*surgery
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cell Transplantation/methods
MH  - Stem Cells/*cytology
MH  - Tyrosine 3-Monooxygenase/metabolism
EDAT- 2008/05/27 09:00
MHDA- 2008/09/10 09:00
CRDT- 2008/05/27 09:00
PHST- 2008/02/29 00:00 [received]
PHST- 2008/04/02 00:00 [revised]
PHST- 2008/04/02 00:00 [accepted]
PHST- 2008/05/27 09:00 [pubmed]
PHST- 2008/09/10 09:00 [medline]
PHST- 2008/05/27 09:00 [entrez]
AID - S0006-8993(08)00878-0 [pii]
AID - 10.1016/j.brainres.2008.04.015 [doi]
PST - ppublish
SO  - Brain Res. 2008 Jun 27;1217:213-20. doi: 10.1016/j.brainres.2008.04.015. Epub 
      2008 Apr 16.