PMID- 18502722
OWN - NLM
STAT- MEDLINE
DCOM- 20080731
LR  - 20210103
IS  - 1121-760X (Print)
IS  - 1121-760X (Linking)
VI  - 52
IP  - 1
DP  - 2008 Jan-Mar
TI  - Flow cytometric detection of circulating dendritic cells in healthy subjects.
PG  - 45-52
AB  - Dendritic cells (DCs) are the key antigen-presenting cells controlling the 
      initiation of the T cell- dependent immune response. Currently, two peripheral 
      blood DC subsets have been identified on the basis of their CD11c expression. The 
      CD11c-negative (CD11c-) DCs (expressing high levels of CD123) are designated as 
      lymphoid-derived DCs (DC2), whereas the CD11c+/CD123- cells, do identify the 
      myeloid-derived DCs (DC1). A growing number of studies have been conducted in 
      recent years on both the quantitative and functional alterations of DCs and their 
      subsets in different pathological conditions. In the present study we assessed, 
      using two different flow cytometric (FCM) techniques, the normal profile of blood 
      DCs in 50 italian adult healthy subjects (M/F: 25/25, median age 42.5 years, 
      range 20-65). The percentage and the absolute number of DCs and their subsets, 
      were obtained starting from whole blood samples in two ways: 1) by calculating 
      the number of DCs when gated as lineage-negative/ HLA-DR+ and identifing the two 
      subsets as CD11c+ (DC1) and CD123+ (DC2) and 2) by using three specific markers: 
      BDCA.1 (CD11c+ high/CD123+ low, myeloid DCs); BDCA.2 (CD11c-/ CD123+high, 
      lymphoid DCs); BDCA.3 (CD11c+low /CD123-, myeloid DCs). Six parameters, 4-color 
      FCM analysis were perfomed with a BD FACSCanto equipment. The mean values of the 
      percentage and of the absolute number were: 0.5+/-0.2% and 30+/-11 cells/microL 
      for DCs; 0.2+/-0.1% and 15+/-6 cells/microL for DC1; 0.2+/-0.1% and 15+/-7 
      cells/microL for DC2. The same values were: 0.2+/-0.1% and 16+/-7 cells/microL 
      for BDCA.1; 0.2+/-0.1% and 12+/-7 cells/microL for BDCA.2; 0.02+/-0.01% and 2+/-1 
      cells/microL for BDCA.3, respectively. Our study confirmes that the two types of 
      FCM analysis are able to identify the DC population. We also provides the first 
      reference values on normal rates and counts of blood DCs in italian adult healthy 
      subjects.
FAU - Rovati, Bianca
AU  - Rovati B
AD  - Medical Oncology IRCCS Foundation S. Matteo University Hospital 27100 Pavia, 
      Italy. flow.cytometry@smatteo.pv.it
FAU - Mariucci, S
AU  - Mariucci S
FAU - Manzoni, M
AU  - Manzoni M
FAU - Bencardino, K
AU  - Bencardino K
FAU - Danova, M
AU  - Danova M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur J Histochem
JT  - European journal of histochemistry : EJH
JID - 9207930
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD/*analysis/immunology
MH  - Dendritic Cells/*cytology/immunology
MH  - Female
MH  - *Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - Male
MH  - Middle Aged
EDAT- 2008/05/27 09:00
MHDA- 2008/08/01 09:00
CRDT- 2008/05/27 09:00
PHST- 2008/05/27 09:00 [pubmed]
PHST- 2008/08/01 09:00 [medline]
PHST- 2008/05/27 09:00 [entrez]
AID - 10.4081/1185 [doi]
PST - ppublish
SO  - Eur J Histochem. 2008 Jan-Mar;52(1):45-52. doi: 10.4081/1185.