PMID- 18502934
OWN - NLM
STAT- MEDLINE
DCOM- 20080731
LR  - 20220318
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 74
IP  - 14
DP  - 2008 Jul
TI  - Competitive oxidation of volatile fatty acids by sulfate- and nitrate-reducing 
      bacteria from an oil field in Argentina.
PG  - 4324-35
LID - 10.1128/AEM.00419-08 [doi]
AB  - Acetate, propionate, and butyrate, collectively referred to as volatile fatty 
      acids (VFA), are considered among the most important electron donors for 
      sulfate-reducing bacteria (SRB) and heterotrophic nitrate-reducing bacteria 
      (hNRB) in oil fields. Samples obtained from a field in the Neuquen Basin, western 
      Argentina, had significant activity of mesophilic SRB, hNRB, and 
      nitrate-reducing, sulfide-oxidizing bacteria (NR-SOB). In microcosms, containing 
      VFA (3 mM each) and excess sulfate, SRB first used propionate and butyrate for 
      the production of acetate, which reached concentrations of up to 12 mM prior to 
      being used as an electron donor for sulfate reduction. In contrast, hNRB used all 
      three organic acids with similar kinetics, while reducing nitrate to nitrite and 
      nitrogen. Transient inhibition of VFA-utilizing SRB was observed with 0.5 mM 
      nitrite and permanent inhibition with concentrations of 1 mM or more. The 
      addition of nitrate to medium flowing into an upflow, packed-bed bioreactor with 
      an established VFA-oxidizing SRB consortium led to a spike of nitrite up to 3 mM. 
      The nitrite-mediated inhibition of SRB led, in turn, to the transient 
      accumulation of up to 13 mM of acetate. The complete utilization of nitrate and 
      the incomplete utilization of VFA, especially propionate, and sulfate indicated 
      that SRB remained partially inhibited. Hence, in addition to lower sulfide 
      concentrations, an increase in the concentration of acetate in the presence of 
      sulfate in waters produced from an oil field subjected to nitrate injection may 
      indicate whether the treatment is successful. The microbial community composition 
      in the bioreactor, as determined by culturing and culture-independent techniques, 
      indicated shifts with an increasing fraction of nitrate. With VFA and sulfate, 
      the SRB genera Desulfobotulus, Desulfotignum, and Desulfobacter as well as the 
      sulfur-reducing Desulfuromonas and the NR-SOB Arcobacter were detected. With VFA 
      and nitrate, Pseudomonas spp. were present. hNRB/NR-SOB from the genus 
      Sulfurospirillum were found under all conditions.
FAU - Grigoryan, Aleksandr A
AU  - Grigoryan AA
AD  - Department of Biological Sciences, University of Calgary, 2500 University Drive 
      NW, Calgary, Alberta T2N 1N4, Canada.
FAU - Cornish, Sabrina L
AU  - Cornish SL
FAU - Buziak, Brenton
AU  - Buziak B
FAU - Lin, Shiping
AU  - Lin S
FAU - Cavallaro, Adriana
AU  - Cavallaro A
FAU - Arensdorf, Joseph J
AU  - Arensdorf JJ
FAU - Voordouw, Gerrit
AU  - Voordouw G
LA  - eng
SI  - GENBANK/EU628133
SI  - GENBANK/EU628134
SI  - GENBANK/EU628135
SI  - GENBANK/EU628136
SI  - GENBANK/EU628137
SI  - GENBANK/EU628138
SI  - GENBANK/EU628139
SI  - GENBANK/EU628140
SI  - GENBANK/EU628141
SI  - GENBANK/EU628142
SI  - GENBANK/EU628143
SI  - GENBANK/EU628144
SI  - GENBANK/EU628145
SI  - GENBANK/EU628146
SI  - GENBANK/EU628147
SI  - GENBANK/EU628148
SI  - GENBANK/EU628149
SI  - GENBANK/EU628150
SI  - GENBANK/EU628151
SI  - GENBANK/EU628152
SI  - GENBANK/EU628153
SI  - GENBANK/EU628154
SI  - GENBANK/EU628155
SI  - GENBANK/EU628156
SI  - GENBANK/EU628157
SI  - GENBANK/EU628158
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080523
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fatty Acids, Volatile)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 0 (Petroleum)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sulfates)
RN  - 0 (Sulfides)
SB  - IM
MH  - Argentina
MH  - Base Sequence
MH  - Bioreactors
MH  - Culture Media
MH  - DNA, Bacterial/isolation & purification
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Fatty Acids, Volatile/*metabolism
MH  - Molecular Sequence Data
MH  - Nitrates/*metabolism
MH  - Nitrites/metabolism
MH  - Oxidation-Reduction
MH  - Petroleum/*microbiology
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/analysis
MH  - Sequence Analysis, DNA
MH  - Sulfates/*metabolism
MH  - Sulfides/metabolism
MH  - Sulfur-Reducing Bacteria/isolation & purification/*metabolism
PMC - PMC2493150
EDAT- 2008/05/27 09:00
MHDA- 2008/08/01 09:00
PMCR- 2009/01/01
CRDT- 2008/05/27 09:00
PHST- 2008/05/27 09:00 [pubmed]
PHST- 2008/08/01 09:00 [medline]
PHST- 2008/05/27 09:00 [entrez]
PHST- 2009/01/01 00:00 [pmc-release]
AID - AEM.00419-08 [pii]
AID - 0419-08 [pii]
AID - 10.1128/AEM.00419-08 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2008 Jul;74(14):4324-35. doi: 10.1128/AEM.00419-08. Epub 
      2008 May 23.