PMID- 18503827
OWN - NLM
STAT- MEDLINE
DCOM- 20080807
LR  - 20191210
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 183
IP  - 2
DP  - 2008 Jun
TI  - Fluorescence in situ hybridization of 12p in germ cell tumors using a bacterial 
      artificial chromosome clone 12p probe on paraffin-embedded tissue: clinical test 
      validation.
PG  - 99-104
LID - 10.1016/j.cancergencyto.2008.02.012 [doi]
AB  - Most germ cell tumors have an isochromosome 12p (detected by metaphase 
      cytogenetics), 12p overrepresentation (detected by fluorescence in situ 
      hybridization [FISH]), or both. Although interphase FISH on paraffin-embedded 
      tissue is a sensitive method of detection of 12p anomalies, use of FISH for 
      clinical diagnostic purposes is not well defined. We describe an interphase FISH 
      assay for detection of increased 12p copy number in germ cell tumors using a 
      bacterial artificial chromosome-derived probe localized to 12p12.1 and a 
      commercially available probe for the centromere of chromosome 12. Twenty-four 
      paraffin-embedded blocks from 14 tumor cases (7 malignant mixed germ cell tumors, 
      2 dysgerminomas, 4 non-germ cell malignancies arising in germ cell tumors, and 1 
      mediastinal adenocarcinoma) and 18 normal controls were studied. Negative 
      controls included normal lymph node, lung, and mediastinal tissue. The signals 
      for 12p and 12cen were counted, and the ratio of the averaged signals was 
      calculated; a ratio of 1.3 was considered positive. All germ cell tumors and 
      non-germ cell malignancies arising in germ cell tumors were positive for 12p 
      overrepresentation. All control cases were negative. Because germ cell tumors may 
      metastasize with non-germ cell tumor morphology, interphase FISH may be helpful 
      in distinguishing de novo malignancy from germ cell tumor recurrence in its 
      various forms.
CI  - (c) 2008 Elsevier Inc.
FAU - Wehle, Danielle
AU  - Wehle D
AD  - Department of Pathology and Laboratory Medicine, The Johns Hopkins University, 
      600 North Wolfe Street, Park Building SB202, Baltimore, MD 21287.
FAU - Yonescu, Raluca
AU  - Yonescu R
FAU - Long, Patricia P
AU  - Long PP
FAU - Gala, Nalini
AU  - Gala N
FAU - Epstein, Jonathan
AU  - Epstein J
FAU - Griffin, Constance A
AU  - Griffin CA
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - *Chromosomes, Artificial, Bacterial
MH  - *Chromosomes, Human, Pair 12
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Neoplasms, Germ Cell and Embryonal/*genetics
MH  - Paraffin Embedding
EDAT- 2008/05/28 09:00
MHDA- 2008/08/08 09:00
CRDT- 2008/05/28 09:00
PHST- 2007/12/03 00:00 [received]
PHST- 2008/02/18 00:00 [revised]
PHST- 2008/02/20 00:00 [accepted]
PHST- 2008/05/28 09:00 [pubmed]
PHST- 2008/08/08 09:00 [medline]
PHST- 2008/05/28 09:00 [entrez]
AID - S0165-4608(08)00148-9 [pii]
AID - 10.1016/j.cancergencyto.2008.02.012 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2008 Jun;183(2):99-104. doi: 
      10.1016/j.cancergencyto.2008.02.012.