PMID- 18504575
OWN - NLM
STAT- MEDLINE
DCOM- 20081002
LR  - 20080625
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 79
IP  - 6
DP  - 2008 Jul
TI  - The macromolecule with antimicrobial activity synthesized by Pseudoalteromonas 
      luteoviolacea strains is an L-amino acid oxidase.
PG  - 925-30
LID - 10.1007/s00253-008-1499-x [doi]
AB  - Two purple pigmented bacterial strains, CPMOR-1 and CPMOR-2, have been newly 
      isolated from the Mediterranean Sea. 16S RNA sequencing and phenotypic 
      characteristics indicate that they belong to the species Pseudoalteromonas 
      luteoviolacea. The synthesis of macromolecules with antimicrobial activity is a 
      capacity described in many strains of this species although the nature of those 
      macromolecules has not been reported up to now. The search for antimicrobial 
      compounds in the two new strains described in this work shows that they 
      synthesize a macromolecule with antimicrobial activity that can be inhibited by 
      catalase, as it had been described in the type strain P. luteoviolacea NCIMB 
      1893(T). This work elucidates the nature of such macromolecule as a novel L-amino 
      acid oxidase (LAO) with broad substrate specificity. The enzyme is most active 
      with Met, Gln, Leu, Phe, Glu, and Trp. In growth media containing those amino 
      acids, the hydrogen peroxide generated by the reaction catalyzed by the LAO 
      mediates its antimicrobial activity.
FAU - Gomez, Daniel
AU  - Gomez D
AD  - Department of Genetics and Microbiology, Faculty of Biology, University of 
      Murcia, Murcia 30100, Spain.
FAU - Espinosa, Elena
AU  - Espinosa E
FAU - Bertazzo, Marcelo
AU  - Bertazzo M
FAU - Lucas-Elio, Patricia
AU  - Lucas-Elio P
FAU - Solano, Francisco
AU  - Solano F
FAU - Sanchez-Amat, Antonio
AU  - Sanchez-Amat A
LA  - eng
SI  - GENBANK/EU158365
SI  - GENBANK/EU158366
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080527
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Amino Acids)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Amino Acids/metabolism
MH  - Anti-Bacterial Agents/chemistry/*metabolism/pharmacology
MH  - Bacterial Proteins/chemistry/genetics/*metabolism/pharmacology
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal/genetics
MH  - Escherichia coli/drug effects
MH  - L-Amino Acid Oxidase/chemistry/genetics/*metabolism/pharmacology
MH  - Molecular Sequence Data
MH  - Pseudoalteromonas/chemistry/*enzymology/genetics/*isolation & purification
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seawater/microbiology
MH  - Substrate Specificity
EDAT- 2008/05/28 09:00
MHDA- 2008/10/03 09:00
CRDT- 2008/05/28 09:00
PHST- 2007/11/15 00:00 [received]
PHST- 2008/04/08 00:00 [accepted]
PHST- 2008/04/08 00:00 [revised]
PHST- 2008/05/28 09:00 [pubmed]
PHST- 2008/10/03 09:00 [medline]
PHST- 2008/05/28 09:00 [entrez]
AID - 10.1007/s00253-008-1499-x [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2008 Jul;79(6):925-30. doi: 10.1007/s00253-008-1499-x. 
      Epub 2008 May 27.