PMID- 18505676
OWN - NLM
STAT- MEDLINE
DCOM- 20080724
LR  - 20081121
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 372
IP  - 4
DP  - 2008 Aug 8
TI  - Coordinate regulation of bovine prion protein gene promoter activity by two Sp1 
      binding site polymorphisms.
PG  - 530-5
LID - 10.1016/j.bbrc.2008.05.085 [doi]
AB  - Relationships between insertion/deletion (Ins/Del) polymorphisms of the bovine 
      prion protein gene (PRNP) promoter and bovine spongiform encephalopathy (BSE) 
      susceptibility have been reported. Our previous study has shown that 
      polymorphisms of -6C-->T included in the specific protein 1 (Sp1) site in the 
      5'-flanking region of bovine PRNP influence the promoter activity of bovine PRNP. 
      The present study shows that 12 and 23bp Ins/Del polymorphisms in the upstream 
      region and an additional polymorphism (-47C-->A) in the Sp1 binding site 
      coordinately affect the promoter activity. Reporter gene assays demonstrated that 
      the bovine PRNP promoter containing -47A and 23bp Del/12bp Ins or 23bp Ins/12bp 
      Ins showed lower promoter activity compared with other haplotypes (23bp Del/12bp 
      Ins or 23bp Ins/12bp Del with -47C) or the wild-type haplotype (23bp Del/12bp Del 
      with -47C). Furthermore, gel shift assays showed that the binding activity of Sp1 
      to the PRNP promoter was influenced by both polymorphisms with corresponding 
      effects on the promoter activity. The coordinate regulation of the bovine PRNP 
      promoter suggests the two Sp1 binding site polymorphisms control Sp1 binding to 
      the PRNP promoter and its activity.
FAU - Xue, Guangai
AU  - Xue G
AD  - Department of Molecular Immunology, School of Agricultural and Life Sciences, 
      University of Tokyo, Bunkyo-ku Yayoi 1-1-1, Tokyo 113-8657, Japan.
FAU - Sakudo, Akikazu
AU  - Sakudo A
FAU - Kim, Chi-kyeong
AU  - Kim CK
FAU - Onodera, Takashi
AU  - Onodera T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080527
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Prions)
RN  - 0 (Sp1 Transcription Factor)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Cattle
MH  - DNA Mutational Analysis
MH  - Electrophoretic Mobility Shift Assay
MH  - Encephalopathy, Bovine Spongiform/*genetics
MH  - *Gene Expression Regulation
MH  - Genes, Reporter
MH  - *Genetic Predisposition to Disease
MH  - Haplotypes
MH  - INDEL Mutation
MH  - Luciferases/genetics
MH  - Molecular Sequence Data
MH  - *Polymorphism, Genetic
MH  - Prions/*genetics
MH  - Promoter Regions, Genetic
MH  - Sp1 Transcription Factor/*metabolism
EDAT- 2008/05/29 09:00
MHDA- 2008/07/25 09:00
CRDT- 2008/05/29 09:00
PHST- 2008/05/09 00:00 [received]
PHST- 2008/05/12 00:00 [accepted]
PHST- 2008/05/29 09:00 [pubmed]
PHST- 2008/07/25 09:00 [medline]
PHST- 2008/05/29 09:00 [entrez]
AID - S0006-291X(08)00956-X [pii]
AID - 10.1016/j.bbrc.2008.05.085 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Aug 8;372(4):530-5. doi: 
      10.1016/j.bbrc.2008.05.085. Epub 2008 May 27.