PMID- 18506422
OWN - NLM
STAT- MEDLINE
DCOM- 20081008
LR  - 20131121
IS  - 0302-8933 (Print)
IS  - 0302-8933 (Linking)
VI  - 190
IP  - 2
DP  - 2008 Aug
TI  - Sulfoacetate released during the assimilation of taurine-nitrogen by 
      Neptuniibacter caesariensis: purification of sulfoacetaldehyde dehydrogenase.
PG  - 159-68
LID - 10.1007/s00203-008-0386-2 [doi]
AB  - Taurine (2-aminoethanesulfonate) is a widespread natural product whose nitrogen 
      moiety was recently shown to be assimilated by bacteria, usually with excretion 
      of an organosulfonate via undefined novel pathways; other data involve 
      transcriptional regulator TauR in taurine metabolism. A screen of genome 
      sequences for TauR with the BLAST algorithm allowed the hypothesis that the 
      marine gammaproteobacterium Neptuniibacter caesariensis MED92 would inducibly 
      assimilate taurine-nitrogen and excrete sulfoacetate. The pathway involved an ABC 
      transporter (TauABC), taurine:pyruvate aminotransferase (Tpa), a novel 
      sulfoacetaldehyde dehydrogenase (SafD) and exporter(s) of sulfoacetate (SafE) 
      (DUF81). Ten candidate genes in two clusters involved three sets of paralogues 
      (for TauR, Tpa and SafE). Inducible Tpa and SafD were detected in cell extracts. 
      SafD was purified 600-fold to homogeneity in two steps. The monomer had a 
      molecular mass of 50 kDa (SDS-PAGE); data from gel filtration chromatography 
      indicated a tetrameric native protein. SafD was specific for sulfoacetaldehyde 
      with a K (m)-value of 0.12 mM. The N-terminal amino acid sequence of SafD 
      confirmed the identity of the safD gene. The eight pathway genes were transcribed 
      inducibly, which indicated expression of the whole hypothetical pathway. We 
      presume that this pathway is one source of sulfoacetate in nature, where this 
      compound is dissimilated by many bacteria.
FAU - Krejcik, Zdenek
AU  - Krejcik Z
AD  - Department of Biology, The University, 78457, Constance, Germany.
FAU - Denger, Karin
AU  - Denger K
FAU - Weinitschke, Sonja
AU  - Weinitschke S
FAU - Hollemeyer, Klaus
AU  - Hollemeyer K
FAU - Paces, Vaclav
AU  - Paces V
FAU - Cook, Alasdair M
AU  - Cook AM
FAU - Smits, Theo H M
AU  - Smits TH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080528
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 0 (Bacterial Proteins)
RN  - 1EQV5MLY3D (Taurine)
RN  - 32797-12-9 (sulfoacetaldehyde)
RN  - EC 1.- (Oxidoreductases)
RN  - GO1N1ZPR3B (Acetaldehyde)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Acetaldehyde/*analogs & derivatives/metabolism
MH  - Bacterial Proteins/chemistry/genetics/*isolation & purification/metabolism
MH  - Gene Expression Regulation, Bacterial
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Nitrogen/*metabolism
MH  - Oceanospirillaceae/*enzymology/genetics/growth & development/metabolism
MH  - Oxidoreductases/chemistry/genetics/*isolation & purification/*metabolism
MH  - Taurine/analogs & derivatives/*metabolism
MH  - Transcription, Genetic
EDAT- 2008/05/29 09:00
MHDA- 2008/10/09 09:00
CRDT- 2008/05/29 09:00
PHST- 2008/03/05 00:00 [received]
PHST- 2008/05/05 00:00 [accepted]
PHST- 2008/04/28 00:00 [revised]
PHST- 2008/05/29 09:00 [pubmed]
PHST- 2008/10/09 09:00 [medline]
PHST- 2008/05/29 09:00 [entrez]
AID - 10.1007/s00203-008-0386-2 [doi]
PST - ppublish
SO  - Arch Microbiol. 2008 Aug;190(2):159-68. doi: 10.1007/s00203-008-0386-2. Epub 2008 
      May 28.