PMID- 18506743
OWN - NLM
STAT- MEDLINE
DCOM- 20081210
LR  - 20131121
IS  - 0269-3879 (Print)
IS  - 0269-3879 (Linking)
VI  - 22
IP  - 10
DP  - 2008 Oct
TI  - Screening and mechanism study of components targeting DNA from the Chinese herb 
      Lonicera japonica by liquid chromatography/mass spectrometry and fluorescence 
      spectroscopy.
PG  - 1164-72
LID - 10.1002/bmc.1040 [doi]
AB  - A method which involves combination of centrifugal ultrafiltration sampling with 
      high-performance liquid chromatography coupled with diode array detection and 
      mass spectrometry (HPLC-DAD-MS) analysis was established for screening bioactive 
      compounds binding to calf thymus deoxyribonucleic acid (ct-DNA) from the extracts 
      of Lonicera japonica. Four compounds were screened out and identified as rutin, 
      quercetin-3-O-glucoside, luteolin-7-O-glucoside and lonicerin, based on the 
      comparison of retention time, UV spectra and MS data with those of standards. The 
      DNA-binding capabilities of the latter three flavonoids were found for the first 
      time. The binding mechanisms of rutin, quercetin-3-O-glucoside and 
      luteolin-7-O-glucoside with ct-DNA at the molecular level were explored using 
      acridine orange (AO) as a fluorescence probe. Groove binding is the most 
      appropriate binding mode of these three flavonoids to DNA, according to 
      ultraviolet absorption and fluorescence spectra, as well as melting temperature 
      (T(m)) curves and viscosity measurements. The binding constants of rutin, 
      quercetin-3-O-glucoside and luteolin-7-O-glucoside with DNA-AO complex were 3.81 
      x 10(3), 3.37 x 10(3) and 5.50 x 10(3) L/mol, respectively.
FAU - Zhou, Jian-Liang
AU  - Zhou JL
AD  - Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), 
      Ministry of Education and Department of Pharmacognosy, China Pharmaceutical 
      University, Nanjing, 210009, People's Republic of China.
FAU - Qian, Zheng-Ming
AU  - Qian ZM
FAU - Luo, Yong-Dong
AU  - Luo YD
FAU - Tang, Dan
AU  - Tang D
FAU - Chen, Hong
AU  - Chen H
FAU - Yi, Ling
AU  - Yi L
FAU - Li, Ping
AU  - Li P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - DNA/*chemistry/drug effects
MH  - Drugs, Chinese Herbal/*metabolism
MH  - Flavonoids/chemistry
MH  - Lonicera/*chemistry
MH  - Mass Spectrometry/*methods
MH  - Osmolar Concentration
MH  - Spectrometry, Fluorescence
MH  - Viscosity
EDAT- 2008/05/29 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/05/29 09:00
PHST- 2008/05/29 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/05/29 09:00 [entrez]
AID - 10.1002/bmc.1040 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2008 Oct;22(10):1164-72. doi: 10.1002/bmc.1040.