PMID- 18506791
OWN - NLM
STAT- MEDLINE
DCOM- 20081027
LR  - 20220223
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 105
IP  - 1
DP  - 2008 Sep 1
TI  - Change in cell shape is required for matrix metalloproteinase-induced 
      epithelial-mesenchymal transition of mammary epithelial cells.
PG  - 25-33
LID - 10.1002/jcb.21821 [doi]
AB  - Cell morphology dictates response to a wide variety of stimuli, controlling cell 
      metabolism, differentiation, proliferation, and death. Epithelial-mesenchymal 
      transition (EMT) is a developmental process in which epithelial cells acquire 
      migratory characteristics, and in the process convert from a "cuboidal" 
      epithelial structure into an elongated mesenchymal shape. We had shown previously 
      that matrix metalloproteinase-3 (MMP3) can stimulate EMT of cultured mouse 
      mammary epithelial cells through a process that involves increased expression of 
      Rac1b, a protein that stimulates alterations in cytoskeletal structure. We show 
      here that cells treated with MMP-3 or induced to express Rac1b spread to cover a 
      larger surface, and that this induction of cell spreading is a requirement of 
      MMP-3/Rac1b-induced EMT. We find that limiting cell spreading, either by 
      increasing cell density or by culturing cells on precisely defined micropatterned 
      substrata, blocks expression of characteristic markers of EMT in cells treated 
      with MMP-3. These effects are not caused by general disruptions in cell signaling 
      pathways, as TGF-beta-induced EMT is not affected by similar limitations on cell 
      spreading. Our data reveal a previously unanticipated cell shape-dependent 
      mechanism that controls this key phenotypic alteration and provide insight into 
      the distinct mechanisms activated by different EMT-inducing agents.
CI  - (c) 2008 Wiley-Liss, Inc.
FAU - Nelson, Celeste M
AU  - Nelson CM
AD  - Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, 
      California 94720, USA. celesten@princeton.edu
FAU - Khauv, Davitte
AU  - Khauv D
FAU - Bissell, Mina J
AU  - Bissell MJ
FAU - Radisky, Derek C
AU  - Radisky DC
LA  - eng
GR  - R37 CA064786/CA/NCI NIH HHS/United States
GR  - CA122086/CA/NCI NIH HHS/United States
GR  - CA128660/CA/NCI NIH HHS/United States
GR  - R21 CA128660/CA/NCI NIH HHS/United States
GR  - R01 CA057621-08/CA/NCI NIH HHS/United States
GR  - R01 CA057621/CA/NCI NIH HHS/United States
GR  - CA64786/CA/NCI NIH HHS/United States
GR  - R01 CA064786-08/CA/NCI NIH HHS/United States
GR  - CA57621/CA/NCI NIH HHS/United States
GR  - R01 CA064786/CA/NCI NIH HHS/United States
GR  - R01 CA122086/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Shape
MH  - Epithelial Cells/*cytology/*enzymology
MH  - Gene Expression Regulation, Enzymologic
MH  - Mammary Glands, Animal/*cytology/*enzymology
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Mesenchymal Stem Cells/cytology/*enzymology
MH  - Mice
MH  - Transforming Growth Factor beta/pharmacology
PMC - PMC2838485
MID - NIHMS160561
EDAT- 2008/05/29 09:00
MHDA- 2008/10/28 09:00
PMCR- 2010/03/15
CRDT- 2008/05/29 09:00
PHST- 2008/05/29 09:00 [pubmed]
PHST- 2008/10/28 09:00 [medline]
PHST- 2008/05/29 09:00 [entrez]
PHST- 2010/03/15 00:00 [pmc-release]
AID - 10.1002/jcb.21821 [doi]
PST - ppublish
SO  - J Cell Biochem. 2008 Sep 1;105(1):25-33. doi: 10.1002/jcb.21821.