PMID- 18508913
OWN - NLM
STAT- MEDLINE
DCOM- 20081222
LR  - 20211020
IS  - 1939-4586 (Electronic)
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 19
IP  - 8
DP  - 2008 Aug
TI  - Chromatin remodeling complexes interact dynamically with a glucocorticoid 
      receptor-regulated promoter.
PG  - 3308-22
AB  - Brahma (BRM) and Brahma-related gene 1 (BRG1) are the ATP-dependent catalytic 
      subunits of the SWI/SNF family of chromatin-remodeling complexes. These complexes 
      are involved in essential processes such as cell cycle, growth, differentiation, 
      and cancer. Using imaging approaches in a cell line that harbors tandem repeats 
      of stably integrated copies of the steroid responsive MMTV-LTR (mouse mammary 
      tumor virus-long terminal repeat), we show that BRG1 and BRM are recruited to the 
      MMTV promoter in a hormone-dependent manner. The recruitment of BRG1 and BRM 
      resulted in chromatin remodeling and decondensation of the MMTV repeat as 
      demonstrated by an increase in the restriction enzyme accessibility and in the 
      size of DNA fluorescence in situ hybridization (FISH) signals. This chromatin 
      remodeling event was concomitant with an increased occupancy of RNA polymerase II 
      and transcriptional activation at the MMTV promoter. The expression of 
      ATPase-deficient forms of BRG1 (BRG1-K-R) or BRM (BRM-K-R) inhibited the 
      remodeling of local and higher order MMTV chromatin structure and resulted in the 
      attenuation of transcription. In vivo photobleaching experiments provided direct 
      evidence that BRG1, BRG1-K-R, and BRM chromatin-remodeling complexes have 
      distinct kinetic properties on the MMTV array, and they dynamically associate 
      with and dissociate from MMTV chromatin in a manner dependent on hormone and a 
      functional ATPase domain. Our data provide a kinetic and mechanistic basis for 
      the BRG1 and BRM chromatin-remodeling complexes in regulating gene expression at 
      a steroid hormone inducible promoter.
FAU - Johnson, Thomas A
AU  - Johnson TA
AD  - Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, 
      National Institutes of Health, Bethesda, MD 20892-5055, USA.
FAU - Elbi, Cem
AU  - Elbi C
FAU - Parekh, Bhavin S
AU  - Parekh BS
FAU - Hager, Gordon L
AU  - Hager GL
FAU - John, Sam
AU  - John S
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20080528
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Chromatin)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Smarca2 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - EC 3.6.1.- (Smarca4 protein, mouse)
RN  - EC 3.6.4.- (DNA Helicases)
SB  - IM
MH  - Adenosine Triphosphate/chemistry
MH  - Animals
MH  - Cell Line, Tumor
MH  - Chromatin/*chemistry/metabolism
MH  - DNA Helicases/*metabolism
MH  - Hydrolysis
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Mammary Tumor Virus, Mouse/metabolism
MH  - Mice
MH  - Models, Biological
MH  - Nuclear Proteins/*metabolism
MH  - *Promoter Regions, Genetic
MH  - RNA Polymerase II/chemistry
MH  - Receptors, Glucocorticoid/*metabolism
MH  - Transcription Factors/*metabolism
PMC - PMC2488306
EDAT- 2008/05/30 09:00
MHDA- 2008/12/23 09:00
PMCR- 2008/09/28
CRDT- 2008/05/30 09:00
PHST- 2008/05/30 09:00 [pubmed]
PHST- 2008/12/23 09:00 [medline]
PHST- 2008/05/30 09:00 [entrez]
PHST- 2008/09/28 00:00 [pmc-release]
AID - E08-02-0123 [pii]
AID - 3367646 [pii]
AID - 10.1091/mbc.e08-02-0123 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2008 Aug;19(8):3308-22. doi: 10.1091/mbc.e08-02-0123. Epub 2008 
      May 28.