PMID- 1850961
OWN - NLM
STAT- MEDLINE
DCOM- 19910604
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 138
IP  - 5
DP  - 1991 May
TI  - Induction of HLA-DR expression on thyroid follicular cells by cytomegalovirus 
      infection in vitro. Evidence for a dual mechanism of induction.
PG  - 1209-23
AB  - Cytomegalovirus (CMV) infection of primary cultures established from human 
      thyroid nodular and normal (paranodular) tissues resulted in induction of human 
      leukocyte antigen (HLA) DR expression on thyroid follicular cells (TFC), as 
      detected by cell-surface immunofluorescence staining with monoclonal antibodies 
      (MAb). Two distinct modalities of induction were observed. The first type 
      occurred in cultures of normal tissue obtained from CMV-seropositive but not 
      seronegative donors, was detected on 30% to 50% of the TFCs, even though the vast 
      majority of these cells failed to show any morphologic or antigenic evidence of 
      individual CMV infection, and was associated with production of gamma-interferon 
      (gamma-IFN) in vitro. The induced molecules displayed the characteristic DR 
      polypeptide profile on immunoprecipitation and electrophoretic analysis. These 
      results demonstrate that CMV infection of normal thyroid cultures may induce DR 
      expression on TFCs in the absence of pre-existing lymphoid infiltrates and 
      suggest that the induction is the result of an in vitro response to CMV by 
      previously sensitized immunocompetent cells present in these primary cultures. 
      Such a response, associated with the release of gamma-IFN, would induce DR 
      expression on neighboring uninfected cells. The second mode of induction occurred 
      in all CMV-infected cultures, regardless of their tissue origin (nodular or 
      normal) or the serologic status of the donors. Up to 50% of infected TFCs at a 
      late stage of infection, having fully developed CMV antigen-positive intranuclear 
      inclusions, also displayed the cell-surface DR-related determinant recognized by 
      one of the four anti-DR MAbs used. This induction was restricted to TFCs, while 
      CMV-infected fibroblastoid cells present in the monolayers were invariably 
      negative. Induction by CMV of major histocompatibility class II antigens on human 
      epithelial cells may have significant implications in the development of normal 
      immune responses against local viral infection, the enhancement of alloimmune 
      rejection of grafted organs, and the generation of organ-specific autoimmune 
      responses.
FAU - Khoury, E L
AU  - Khoury EL
AD  - Division of Oral Biology, University of California, San Francisco 94115.
FAU - Pereira, L
AU  - Pereira L
FAU - Greenspan, F S
AU  - Greenspan FS
LA  - eng
GR  - DK36164/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal/immunology
MH  - Cells, Cultured
MH  - Cytomegalovirus/isolation & purification/*physiology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation, Viral/*physiology
MH  - HLA-DR Antigens/genetics/immunology/*metabolism
MH  - Histocompatibility Antigens Class II/genetics/immunology/metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Male
MH  - Middle Aged
MH  - Thyroid Gland/immunology/*metabolism/pathology
PMC - PMC1886009
EDAT- 1991/05/01 00:00
MHDA- 1991/05/01 00:01
PMCR- 1991/11/01
CRDT- 1991/05/01 00:00
PHST- 1991/05/01 00:00 [pubmed]
PHST- 1991/05/01 00:01 [medline]
PHST- 1991/05/01 00:00 [entrez]
PHST- 1991/11/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1991 May;138(5):1209-23.