PMID- 18510047
OWN - NLM
STAT- MEDLINE
DCOM- 20080612
LR  - 20220409
IS  - 0017-0011 (Print)
IS  - 0017-0011 (Linking)
VI  - 79
IP  - 1
DP  - 2008 Jan
TI  - [Expression of tumor necrosis factor-alpha (TNF-alpha) on peritoneal fluid 
      mononuclear cells in women with endometriosis].
PG  - 31-5
AB  - OBJECTIVES: Tumor necrosis factor-alpha (TNF-alpha) plays a key role in the 
      processes underlying the development of pelvic endometriosis. TNF-alpha acts on 
      target cells via two receptors: TNFR1(p55) and TNFR2(p75). Depending on cell type 
      and its activation state, ligand binding to TNF-alpha may induce activation and 
      proliferation of the cells or promote apoptosis. The aim of our study has been to 
      evaluate the expression of TNFR1 and TNFR2 on peritoneal fluid macrophages and T 
      lymphocytes derived from women with endometriosis. MATERIAL AND METHODS: The 
      study group consisted of 22 patients with endometriosis (stage I and II rAFS). 14 
      patients with benign, non-inflammatory ovarian tumors composed the reference 
      group. Mononuclear cells have been isolated from peritoneal fluid, obtained 
      during laparoscopy. The expression of TNFR1 and TNFR2 proteins has been evaluated 
      by means of flow cytometry, using monoclonal antibodies against CD120a, CD120b, 
      CD3 and CD14. RESULTS: The percentage of peritoneal fluid macrophages revealing 
      the expression of TNFR1 and TNFR2 proteins has been higher in patients with 
      endometriosis, in comparison with control group (22.6+/-5.3% vs. 6.8+/-1,8%; 
      p=0.03 and 29.3+/-2.3% vs. 8.8+/-1.8%; p=0.01, respectively). The percentage of T 
      lymphocytes with the expression of TNFR1 and TNFR2 has been similar in 
      endometriosis and control group. CONCLUSION: Higher percentage of peritoneal 
      fluid macrophages expressing TNFR1 and TNFR2 proteins in endometriosis suggests 
      dependence of these cells on TNF-alpha stimulation. Changes in TNF receptors 
      distribution on PF macrophages, but not lymphocyte, may play its role in the 
      pathogenesis of endometriosis.
FAU - Gogacz, Marek
AU  - Gogacz M
AD  - II Katedra i Klinika Ginekologii Akademii Medycznej im. Prof. Feliksa 
      Skubiszewskiego w Lublinie, Lublin. gogacz@yahoo.com
FAU - Bogusiewicz, Michal
AU  - Bogusiewicz M
FAU - Putowski, Lechoslaw
AU  - Putowski L
FAU - Adamiak, Aneta
AU  - Adamiak A
FAU - Wertel, Iwona
AU  - Wertel I
FAU - Jakowicki, Jerzy A
AU  - Jakowicki JA
FAU - Rechberger, Tomasz
AU  - Rechberger T
LA  - pol
PT  - English Abstract
PT  - Journal Article
TT  - Ekspresja receptorow dla czynnika martwicy guza-alpha (TNF-alpha) na 
      jednojadrzastych komorkach immunokompetentnych plynu otrzewnowego u kobiet z 
      endometrioza.
PL  - Poland
TA  - Ginekol Pol
JT  - Ginekologia polska
JID - 0374641
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type II)
SB  - IM
MH  - Adult
MH  - Ascitic Fluid/chemistry/*metabolism
MH  - Case-Control Studies
MH  - Endometriosis/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Macrophages, Peritoneal/*metabolism
MH  - Middle Aged
MH  - Receptors, Tumor Necrosis Factor, Type I/*metabolism
MH  - Receptors, Tumor Necrosis Factor, Type II/*metabolism
MH  - Reference Values
MH  - T-Lymphocytes/*metabolism
MH  - Women's Health
EDAT- 2008/05/31 09:00
MHDA- 2008/06/13 09:00
CRDT- 2008/05/31 09:00
PHST- 2008/05/31 09:00 [pubmed]
PHST- 2008/06/13 09:00 [medline]
PHST- 2008/05/31 09:00 [entrez]
PST - ppublish
SO  - Ginekol Pol. 2008 Jan;79(1):31-5.