PMID- 18510313
OWN - NLM
STAT- MEDLINE
DCOM- 20080729
LR  - 20080625
IS  - 1554-8937 (Electronic)
IS  - 1554-8929 (Linking)
VI  - 3
IP  - 6
DP  - 2008 Jun 20
TI  - Cell-based fluorescent indicator to visualize brain-derived neurotrophic factor 
      secreted from living neurons.
PG  - 352-8
LID - 10.1021/cb800052v [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a polypeptide that is secreted from 
      neurons. Although there is mounting evidence that BDNF regulates neuronal 
      development and synaptic plasticity, BDNF secretion has remained unclear due to 
      lack of appropriate methods for the analysis of its dynamics. To visualize BDNF 
      secretion from neurons, here we have developed a cell-based fluorescent indicator 
      for BDNF. We showed that the present cell-based fluorescent indicator, named 
      "Bescell", has high selectivity to BDNF and detects picomolar concentrations of 
      BDNF (detection limit of 60 pM). Bescell has visualized endogenous BDNF secreted 
      from hippocampal neurons. It thus provides a powerful tool for the analysis of 
      BDNF secretion from living neurons.
FAU - Nakajima, Takahiro
AU  - Nakajima T
FAU - Sato, Moritoshi
AU  - Sato M
FAU - Akaza, Naoko
AU  - Akaza N
FAU - Umezawa, Yoshio
AU  - Umezawa Y
LA  - eng
PT  - Letter
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - ACS Chem Biol
JT  - ACS chemical biology
JID - 101282906
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA, Complementary)
RN  - 0 (Luminescent Agents)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Biosensing Techniques/*methods
MH  - *Brain-Derived Neurotrophic Factor/analysis/metabolism
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA, Complementary/genetics
MH  - Female
MH  - Fluorescence Resonance Energy Transfer/*methods
MH  - *Hippocampus/metabolism/pathology
MH  - Humans
MH  - Luminescent Agents/*chemistry
MH  - *Neurons/cytology/metabolism
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 2008/05/31 09:00
MHDA- 2008/07/30 09:00
CRDT- 2008/05/31 09:00
PHST- 2008/05/31 09:00 [pubmed]
PHST- 2008/07/30 09:00 [medline]
PHST- 2008/05/31 09:00 [entrez]
AID - 10.1021/cb800052v [doi]
PST - ppublish
SO  - ACS Chem Biol. 2008 Jun 20;3(6):352-8. doi: 10.1021/cb800052v.