PMID- 18511576
OWN - NLM
STAT- MEDLINE
DCOM- 20080904
LR  - 20221207
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 84
IP  - 2
DP  - 2008 Aug
TI  - Commensal bacteria trigger a full dendritic cell maturation program that promotes 
      the expansion of non-Tr1 suppressor T cells.
PG  - 468-76
LID - 10.1189/jlb.0108017 [doi]
AB  - Dendritic cells (DCs) orchestrate the immune response establishing immunity 
      versus tolerance. These two opposite functions may be dictated by DC maturation 
      status with maturity linked to immunogenicity. DCs directly interact with 
      trillions of noninvasive intestinal bacteria in vivo, a process that contributes 
      to gut homeostasis. We here evaluated the maturation program elicited in human 
      DCs by direct exposure to commensal-related bacteria (CB) in the absence of 
      inflammatory signals. We showed that eight gram(+) and gram(-) CB strains 
      up-regulated costimulatory molecule expression in DCs and provoked a chemokine 
      receptor switch similar to that activated by gram(+) pathogens. CB strains may be 
      classified into three groups according to DC cytokine release: high IL-12 and low 
      IL-10; low IL-12 and high IL-10; and low IL-12 and IL-10. All CB-treated DCs 
      produced IL-1beta and IL-6 and almost no TGF-beta. Yet, CB instructed DCs to 
      convert naive CD4+ T cells into hyporesponsive T cells that secreted low or no 
      IFN-gamma, IL-10, and IL-17 and instead, displayed suppressor function. These 
      data demonstrate that phenotypic DC maturation combined to an appropriate 
      cytokine profile is insufficient to warrant Th1, IL-10-secreting T regulatory 
      Type 1 (Tr1), or Th17 polarization. We propose that commensal flora and as such, 
      probiotics manipulate DCs by a yet-unidentified pathway to enforce gut tolerance.
FAU - Baba, Nobuyasu
AU  - Baba N
AD  - Immunoregulation Laboratory, Centre Hospitalier de l'Universite de Montreal 
      Research Center, University of Montreal, Quebec, Canada.
FAU - Samson, Sandrine
AU  - Samson S
FAU - Bourdet-Sicard, Raphaelle
AU  - Bourdet-Sicard R
FAU - Rubio, Manuel
AU  - Rubio M
FAU - Sarfati, Marika
AU  - Sarfati M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080529
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Antibody Formation
MH  - Cell Culture Techniques
MH  - Dendritic Cells/*immunology/*microbiology
MH  - Flow Cytometry
MH  - Humans
MH  - Immunosuppression Therapy
MH  - Interleukin-10/metabolism
MH  - Interleukin-12/metabolism
MH  - Lacticaseibacillus casei/*immunology
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Tissue Expansion
EDAT- 2008/05/31 09:00
MHDA- 2008/09/05 09:00
CRDT- 2008/05/31 09:00
PHST- 2008/05/31 09:00 [pubmed]
PHST- 2008/09/05 09:00 [medline]
PHST- 2008/05/31 09:00 [entrez]
AID - jlb.0108017 [pii]
AID - 10.1189/jlb.0108017 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2008 Aug;84(2):468-76. doi: 10.1189/jlb.0108017. Epub 2008 May 29.