PMID- 18512875
OWN - NLM
STAT- MEDLINE
DCOM- 20080815
LR  - 20211020
IS  - 0743-7463 (Print)
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 24
IP  - 13
DP  - 2008 Jun 1
TI  - Exercising spatiotemporal control of cell attachment with optically transparent 
      microelectrodes.
PG  - 6837-44
LID - 10.1021/la800231e [doi]
AB  - This paper describes a novel approach of controlling cell-surface interactions 
      through an electrochemical "switching" of biointerfacial properties of optically 
      transparent microelectrodes. The indium tin oxide (ITO) microelectrodes, 
      fabricated on glass substrates, were modified with poly(ethylene glycol) (PEG) 
      silane to make glass and ITO regions resistant to protein and cell adhesion. 
      Cyclic voltammetry, with potassium ferricyanide serving as a redox reporter 
      molecule, was used to monitor electron transfer across the electrolyte-ITO 
      interface. PEG silane modification of ITO correlated with diminished electron 
      transfer, judged by the disappearance of ferricyanide redox activity. 
      Importantly, application of reductive potential (-1.4 V vs Ag/AgCl reference) 
      corresponded with reappearance of typical ferricyanide redox peaks, thus pointing 
      to desorption of an insulating PEG silane layer. Time-of-flight secondary ion 
      mass spectrometry (ToF-SIMS) characterization of the silanized ITO surfaces after 
      electrical stimulation indicated complete removal of the silane layer. 
      Significantly, electrical stimulation allowed to "switch" chosen electrodes from 
      nonfouling to protein-adhesive while leaving other ITO and glass regions 
      protected by a nonfouling PEG silane layer. The spatial and temporal control of 
      biointerfacial properties afforded by our approach was utilized to micropattern 
      proteins and cells and to construct micropatterned co-cultures. In the future, 
      control of the biointerfacial properties afforded by this novel approach may 
      allow the organization of multiple cell types into precise geometric 
      configurations in order to create better in vitro mimics of cellular complexity 
      of the native tissues.
FAU - S Shah, Sunny
AU  - S Shah S
AD  - Department of Biomedical Engineering, University of California, Davis, CA, USA.
FAU - Lee, Ji Youn
AU  - Lee JY
FAU - Verkhoturov, Stanislav
AU  - Verkhoturov S
FAU - Tuleuova, Nazgul
AU  - Tuleuova N
FAU - Schweikert, Emile A
AU  - Schweikert EA
FAU - Ramanculov, Erlan
AU  - Ramanculov E
FAU - Revzin, Alexander
AU  - Revzin A
LA  - eng
GR  - R21 DK073901/DK/NIDDK NIH HHS/United States
GR  - R21 DK073901-02/DK/NIDDK NIH HHS/United States
GR  - DK073901/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080530
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
SB  - IM
MH  - Cell Adhesion
MH  - Cell Culture Techniques/*methods
MH  - Cell Line, Tumor
MH  - Humans
MH  - Mass Spectrometry
MH  - Microelectrodes
MH  - Molecular Structure
PMC - PMC3677040
MID - NIHMS345235
EDAT- 2008/06/03 09:00
MHDA- 2008/08/16 09:00
PMCR- 2013/06/09
CRDT- 2008/06/03 09:00
PHST- 2008/06/03 09:00 [pubmed]
PHST- 2008/08/16 09:00 [medline]
PHST- 2008/06/03 09:00 [entrez]
PHST- 2013/06/09 00:00 [pmc-release]
AID - 10.1021/la800231e [doi]
PST - ppublish
SO  - Langmuir. 2008 Jun 1;24(13):6837-44. doi: 10.1021/la800231e. Epub 2008 May 30.