PMID- 18513162
OWN - NLM
STAT- MEDLINE
DCOM- 20080829
LR  - 20080625
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 17
IP  - 3
DP  - 2008 Jun
TI  - Epithelial-mesenchymal cell ratios can determine the crown morphogenesis of 
      dental pulp stem cells.
PG  - 475-82
LID - 10.1089/scd.2007.0120 [doi]
AB  - Although dental pulp stem cells (DPSC) have been isolated from adult dental pulp 
      tissues, knowledge on how to use them to make teeth lags behind. To date, little 
      is known about the effects of epithelial-mesenchymal cell ratios on the 
      bioengineered odontogenesis mediated by DPSCs. In this study, we investigated the 
      effects of apical bud cells (ABC) from dental epithelial stem cell niche of rat 
      incisors on the differentiation and morphogenesis of molar DPSCs at different 
      proportions (DPSC/ABC cell ratios=1:10, 1:3, 1:1, 3:1, 10:1, respectively). In 
      vitro mixed DPSCs/ABCs at 1:1, 1:3, and 3:1 ratios displayed several crucial 
      characteristics of odontoblast/ameloblast lineages, as indicated by accelerated 
      mineralization, upregulated alkaline phosphatase activity, protein/gene 
      expression for dentin sialophosphoprotein and ameloblastin. In vivo 
      transplantation of reassociated DPSC and ABC pellets at different ratios was also 
      carried out. Histological analyses demonstrated that only DPSC/ABC recombinants 
      at 1:1 ratio generated typical molar crown-shaped structures, whereas 
      recombinations at other ratios presented an atypical crown morphogenesis with 
      unbalanced distribution of amelogenesis and dentinogenesis. Together, these 
      findings revealed that the proportions of dental epithelial and mesenchymal cell 
      populations can determine the odontogenic differentiation of DPSCs/ABCs in vitro 
      as well as the bioengineered tooth morphogenesis in vivo.
FAU - Yu, Jinghua
AU  - Yu J
AD  - Department of Endodontics, School of Stomatology, Nanjing Medical University, 
      Nanjing, Jiangsu, China.
FAU - Jin, Fang
AU  - Jin F
FAU - Deng, Zhihong
AU  - Deng Z
FAU - Li, Yuanfei
AU  - Li Y
FAU - Tang, Liang
AU  - Tang L
FAU - Shi, Junnan
AU  - Shi J
FAU - Jin, Yan
AU  - Jin Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Cell Count
MH  - Coculture Techniques
MH  - Dental Pulp/*cytology
MH  - Epithelial Cells/*cytology
MH  - Immunohistochemistry
MH  - Mesoderm/*cytology
MH  - *Morphogenesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cells/*cytology/enzymology
MH  - Tooth Crown/*embryology
EDAT- 2008/06/03 09:00
MHDA- 2008/08/30 09:00
CRDT- 2008/06/03 09:00
PHST- 2008/06/03 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/06/03 09:00 [entrez]
AID - 10.1089/scd.2007.0120 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2008 Jun;17(3):475-82. doi: 10.1089/scd.2007.0120.