PMID- 18513372
OWN - NLM
STAT- MEDLINE
DCOM- 20081103
LR  - 20211020
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 106
IP  - 5
DP  - 2008 Sep
TI  - Expression of E-FABP in PC12 cells increases neurite extension during 
      differentiation: involvement of n-3 and n-6 fatty acids.
PG  - 2015-29
LID - 10.1111/j.1471-4159.2008.05507.x [doi]
AB  - Epidermal fatty acid-binding protein (E-FABP), a member of the family of FABPs, 
      exhibits a robust expression in neurons during axonal growth in development and 
      in nerve regeneration following nerve injury. This study examines the impact of 
      E-FABP expression in normal neurite extension in differentiating pheochromocytoma 
      cell (PC12) cultures supplemented with selected long chain free fatty acids 
      (LCFFA). We found that E-FABP binds to a broad range of saturated and unsaturated 
      LCFFAs, including those with potential interest for neuronal differentiation and 
      axonal growth such as C22:6n-3 docosahexaenoic acid (DHA), C20:5n-3 
      eicosapentaenoic acid (EPA), and C20:4n-6 arachidonic acid (ARA). PC12 cells 
      exposed to nerve growth factor (NGFDPC12) exhibit high E-FABP expression that is 
      blocked by mitogen-activated protein kinase kinase (MEK) inhibitor U0126. Nerve 
      growth factor-differentiated pheochromocytoma cells (NGFDPC12) antisense clones 
      (NGFDPC12-AS) which exhibit low E-FABP expression have fewer/shorter neurites 
      than cells transfected with vector only or NGFDPC12 sense cells (NGFDPC12-S). 
      Replenishing NGFDPC12-AS cells with biotinylated recombinant E-FABP 
      (biotin-E-FABP) protein restores normal neurite outgrowth. Cellular localization 
      of biotin-E-FABP in NGFDPC12 was detected mostly in the cytoplasm and in the 
      nuclear region. Treatment of NGFDPC12 with DHA, EPA, or ARA further enhances 
      neurite length but it does not trigger further induction of TrkA or MEK 
      phosphorylation or E-FABP mRNA observed in differentiating PC12 cells without 
      LCFFA supplementation. Significantly, DHA and EPA neurite stimulating effects are 
      higher in NGFDPC12-S than in NGFDPC12-AS cells. These findings are consistent 
      with the scenario that neurite extension of differentiating PC12 cells, including 
      further stimulation by DHA and EPA, requires sufficient cellular levels of 
      E-FABP.
FAU - Liu, Jo-Wen
AU  - Liu JW
AD  - Center for Health Disparities and Molecular Medicine, Department of Basic 
      Science, Loma Linda University, California 92350, USA.
FAU - Almaguel, Frankis G
AU  - Almaguel FG
FAU - Bu, Liming
AU  - Bu L
FAU - De Leon, Daisy D
AU  - De Leon DD
FAU - De Leon, Marino
AU  - De Leon M
LA  - eng
GR  - P20 MD001632/MD/NIMHD NIH HHS/United States
GR  - P20 MD006988/MD/NIMHD NIH HHS/United States
GR  - 5R25GM060507/GM/NIGMS NIH HHS/United States
GR  - P20 MD001632-04/MD/NIMHD NIH HHS/United States
GR  - R25 GM060507/GM/NIGMS NIH HHS/United States
GR  - R25 GM060507-02/GM/NIGMS NIH HHS/United States
GR  - R25 GM060507-01A1/GM/NIGMS NIH HHS/United States
GR  - R25 GM060507-08/GM/NIGMS NIH HHS/United States
GR  - 5P20MD001632/MD/NIMHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080530
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Eye Proteins)
RN  - 0 (Fabp5 protein, rat)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Fatty Acids, Omega-6)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 1)
SB  - IM
MH  - Animals
MH  - Cell Compartmentation/physiology
MH  - Cell Differentiation/drug effects/*physiology
MH  - Docosahexaenoic Acids/metabolism/pharmacology
MH  - Eicosapentaenoic Acid/metabolism/pharmacology
MH  - Eye Proteins/genetics/*metabolism
MH  - Fatty Acid-Binding Proteins/genetics/*metabolism
MH  - Fatty Acids, Omega-3/*metabolism/pharmacology
MH  - Fatty Acids, Omega-6/*metabolism/pharmacology
MH  - Lipid Metabolism/drug effects/*physiology
MH  - MAP Kinase Kinase 1/antagonists & inhibitors/metabolism
MH  - Nerve Growth Factor/metabolism/pharmacology
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Neurites/drug effects/*metabolism/ultrastructure
MH  - Oligodeoxyribonucleotides, Antisense/pharmacology
MH  - PC12 Cells
MH  - Protein Binding/drug effects/physiology
MH  - Rats
MH  - Recombinant Fusion Proteins/pharmacology
PMC - PMC2785489
MID - NIHMS155546
EDAT- 2008/06/03 09:00
MHDA- 2008/11/04 09:00
PMCR- 2009/11/30
CRDT- 2008/06/03 09:00
PHST- 2008/06/03 09:00 [pubmed]
PHST- 2008/11/04 09:00 [medline]
PHST- 2008/06/03 09:00 [entrez]
PHST- 2009/11/30 00:00 [pmc-release]
AID - JNC5507 [pii]
AID - 10.1111/j.1471-4159.2008.05507.x [doi]
PST - ppublish
SO  - J Neurochem. 2008 Sep;106(5):2015-29. doi: 10.1111/j.1471-4159.2008.05507.x. Epub 
      2008 May 30.