PMID- 18513409
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20100609
LR  - 20211020
IS  - 1749-8546 (Electronic)
IS  - 1749-8546 (Linking)
VI  - 3
DP  - 2008 May 30
TI  - An optimized high-performance liquid chromatography (HPLC) method for 
      benzoylmesaconine determination in Radix Aconiti Lateralis Preparata (Fuzi, 
      aconite roots) and its products.
PG  - 6
LID - 10.1186/1749-8546-3-6 [doi]
AB  - BACKGROUND: Benzoylmesaconine (BMA) is the main Aconitum alkaloid in Radix 
      Aconiti Lateralis Preparata (Fuzi, aconite roots) with potent pharmacological 
      activities, such as analgesia and anti-inflammation. The present study developed 
      a simple and reliable method using BMA as a marker compound for the quality 
      control of processed aconite roots and their products. METHODS: After extraction, 
      a high-performance liquid chromatography (HPLC) determination of BMA was 
      conducted on a RP-C18 column by gradient elution with acetonitrile and aqueous 
      phase, containing 0.1% phosphoric acid adjusted with triethylamine to pH 3.0. 
      RESULTS: A distinct peak profile was obtained and separation of BMA was achieved. 
      Method validation showed that the relative standard deviations (RSDs) of the 
      precision of BMA in all intra-day and inter-day assays were less than 1.36%, and 
      that the average recovery rate was 96.95%. Quantitative analysis of BMA showed 
      that the content of BMA varied significantly in processed aconite roots and their 
      products. CONCLUSION: This HPLC method using BMA as a marker compound is 
      applicable to the quality control of processed aconite roots and their products.
FAU - Xie, Ying
AU  - Xie Y
AD  - School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, PR 
      China. 04415930@hkbu.edu.hk
FAU - Zhou, Hua
AU  - Zhou H
FAU - Wong, Yuen Fan
AU  - Wong YF
FAU - Liu, Zhongqiu
AU  - Liu Z
FAU - Xu, Hongxi
AU  - Xu H
FAU - Jiang, Zhihong
AU  - Jiang Z
FAU - Liu, Liang
AU  - Liu L
LA  - eng
PT  - Journal Article
DEP - 20080530
PL  - England
TA  - Chin Med
JT  - Chinese medicine
JID - 101265109
PMC - PMC2430698
EDAT- 2008/06/03 09:00
MHDA- 2008/06/03 09:01
PMCR- 2008/05/30
CRDT- 2008/06/03 09:00
PHST- 2006/08/08 00:00 [received]
PHST- 2008/05/30 00:00 [accepted]
PHST- 2008/06/03 09:00 [pubmed]
PHST- 2008/06/03 09:01 [medline]
PHST- 2008/06/03 09:00 [entrez]
PHST- 2008/05/30 00:00 [pmc-release]
AID - 1749-8546-3-6 [pii]
AID - 10.1186/1749-8546-3-6 [doi]
PST - epublish
SO  - Chin Med. 2008 May 30;3:6. doi: 10.1186/1749-8546-3-6.