PMID- 18514625
OWN - NLM
STAT- MEDLINE
DCOM- 20080724
LR  - 20131121
IS  - 0002-9149 (Print)
IS  - 0002-9149 (Linking)
VI  - 101
IP  - 11A
DP  - 2008 Jun 2
TI  - Oral amino acid supplementation counteracts age-induced sarcopenia in elderly 
      rats.
PG  - 35E-41E
LID - 10.1016/j.amjcard.2008.02.079 [doi]
AB  - We investigated the effects of a specific mixture of amino acid (AA) supplements 
      on the adaptation changes induced by aging in the soleus muscle of rats. Male 
      Wistar rats were divided into 3 groups (n = 5 each): young control (YO), 3 months 
      of age; elderly control (EL), 18 months of age; and elderly orally supplemented 
      with an AA mixture (EL-AA), 18 months of age, given as 0.1 g/kg per day in 
      drinking water for 8 weeks. Myosin heavy chain (MHC) composition was analyzed in 
      all muscles. The total fiber number and fiber cross-sectional area of types 1 and 
      2A fibers were also measured in immunostained sections of the soleus muscle. The 
      ratios between the sarcomere volume (Vsar) and the total volume (Vtot) and single 
      muscle fibers were studied by electron microscopy. The expression of total and 
      phosphorylated serine/threonine protein kinase mammalian target of rapamycin 
      (mTOR), a potent regulator of messenger RNA translation initiation, was also 
      determined in all groups. Aging was associated with an overall shift toward the 
      expression of a slower MHC phenotype, atrophy of fast and slow fibers, a 
      significant decrease in Vtot/Vsar, and no changes in total fiber number. AA 
      supplementation antagonized the effects of aging. A shift toward the expression 
      of faster MHC isoforms was observed. Fiber atrophy appeared to be partly 
      counteracted by the AA supplements; we noted an increase in cross-sectional area 
      fibers and Vtot/Vsar in EL-AAs. Total and phosphorylated mTOR expression appeared 
      to decrease in EL and was restored by the AA supplements. Collectively, these 
      results suggest that aging-induced muscle adaptations can be partly restored by 
      AA supplementation. An mTOR signal pathway may mediate the effects on fiber 
      trophism.
FAU - Pansarasa, Orietta
AU  - Pansarasa O
AD  - Department of Experimental Medicine, Human Physiology Unit, University of Pavia, 
      Pavia, Italy.
FAU - Flati, Vincenzo
AU  - Flati V
FAU - Corsetti, Giovanni
AU  - Corsetti G
FAU - Brocca, Lorenza
AU  - Brocca L
FAU - Pasini, Evasio
AU  - Pasini E
FAU - D'Antona, Giuseppe
AU  - D'Antona G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Cardiol
JT  - The American journal of cardiology
JID - 0207277
RN  - 0 (Amino Acids)
RN  - 0 (Muscle Proteins)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Aging/pathology/physiology
MH  - Amino Acids/*administration & dosage
MH  - Animals
MH  - Blotting, Western
MH  - *Dietary Supplements
MH  - Immunohistochemistry
MH  - Male
MH  - Muscle Fibers, Skeletal/drug effects/pathology
MH  - Muscle Proteins/*drug effects/*metabolism
MH  - Muscle, Skeletal/*drug effects/*pathology
MH  - Myosin Heavy Chains/drug effects/physiology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2008/07/02 09:00
MHDA- 2008/07/25 09:00
CRDT- 2008/07/02 09:00
PHST- 2008/07/02 09:00 [pubmed]
PHST- 2008/07/25 09:00 [medline]
PHST- 2008/07/02 09:00 [entrez]
AID - S0002-9149(08)00394-9 [pii]
AID - 10.1016/j.amjcard.2008.02.079 [doi]
PST - ppublish
SO  - Am J Cardiol. 2008 Jun 2;101(11A):35E-41E. doi: 10.1016/j.amjcard.2008.02.079.