PMID- 18514942
OWN - NLM
STAT- MEDLINE
DCOM- 20081006
LR  - 20181201
IS  - 0168-1656 (Print)
IS  - 0168-1656 (Linking)
VI  - 135
IP  - 3
DP  - 2008 Jun 30
TI  - Expression in yeast of secreted lignin peroxidase with improved 
      2,4-dichlorophenol degradability by DNA shuffling.
PG  - 241-6
LID - 10.1016/j.jbiotec.2008.04.007 [doi]
AB  - Lignin peroxidase (LiP) from Phanerochaete chrysosporium was shown to mineralize 
      a variety of recalcitrant aromatic compounds and oxidize a number of polycyclic 
      aromatic and phenolic compounds. The major problem of the wild type LiP is that 
      it can be inactivated by excess H(2)O(2) and high concentrations of aromatic 
      compounds. We applied a directed evolution technique coupled with a rapid 
      colorimetric screening method to obtain mutant genes with improved H(2)O(2) 
      stability and polychlorinated phenol degradability, and they were successfully 
      expressed as the secretive LiPs in recombinant Saccharomyces cerevisiae. The 
      resulting variants showed approximately 1.6-fold improved 2,4-dichlorophenol 
      (2,4-DCP) degradation activity and stability against H(2)O(2) compared with the 
      parent strain. The kinetic properties of the variants toward 2,4-DCP and H(2)O(2) 
      were also increased compared with the wild type for all three mutants studied. 
      Amino acid sequence analysis indicated that the greatest number of amino acid 
      substitutions was located near the surface or Ca(2+) binding sites of the enzyme.
FAU - Ryu, Kang
AU  - Ryu K
AD  - Catholic Research Institute of Medical Science, College of Medicine, The Catholic 
      University of Korea, 505 Banpo-dong, Seocho-gu, Seoul, Korea.
FAU - Kang, Jung Hye
AU  - Kang JH
FAU - Wang, Lishi
AU  - Wang L
FAU - Lee, E K
AU  - Lee EK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080418
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (Chlorophenols)
RN  - 0 (Culture Media)
RN  - EC 1.11.1.- (Peroxidases)
RN  - EC 1.11.1.- (lignin peroxidase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Amino Acid Substitution
MH  - Biodegradation, Environmental
MH  - Chlorophenols/*metabolism
MH  - Colorimetry
MH  - Culture Media
MH  - *DNA Shuffling
MH  - Kinetics
MH  - Mutation/genetics
MH  - Peroxidases/*metabolism
MH  - Phanerochaete/*enzymology
MH  - Protein Structure, Secondary
MH  - Saccharomyces cerevisiae/*genetics
EDAT- 2008/06/03 09:00
MHDA- 2008/10/07 09:00
CRDT- 2008/06/03 09:00
PHST- 2007/12/18 00:00 [received]
PHST- 2008/04/02 00:00 [revised]
PHST- 2008/04/07 00:00 [accepted]
PHST- 2008/06/03 09:00 [pubmed]
PHST- 2008/10/07 09:00 [medline]
PHST- 2008/06/03 09:00 [entrez]
AID - S0168-1656(08)00153-3 [pii]
AID - 10.1016/j.jbiotec.2008.04.007 [doi]
PST - ppublish
SO  - J Biotechnol. 2008 Jun 30;135(3):241-6. doi: 10.1016/j.jbiotec.2008.04.007. Epub 
      2008 Apr 18.