PMID- 18515832
OWN - NLM
STAT- MEDLINE
DCOM- 20080806
LR  - 20240109
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Linking)
VI  - 59
IP  - 9
DP  - 2008
TI  - Early events induced by chitosan on plant cells.
PG  - 2317-24
LID - 10.1093/jxb/ern096 [doi]
AB  - Chitosan (a polymer of beta-1,4-glucosamine residues) is a deacetylated 
      derivative of chitin which presents antifungal properties and acts as a potent 
      elicitor of plant resistance against fungal pathogens. Attention was focused in 
      this study on the chitosan-induced early events in the elicitation chain. Thus, 
      it was shown that chitosan triggered in a dose-dependent manner rapid membrane 
      transient depolarization of Mimosa pudica motor cells and, correlatively, a 
      transient rise of pH in the incubation medium of pulvinar tissues. By using 
      plasma membrane vesicles (PMVs), it was specified that a primary site of action 
      of the compound is the plasma membrane H(+)-ATPase as shown by its inhibitory 
      effect on the proton pumping and the catalytic activity of the enzyme up to 250 
      microg ml(-1). As a consequence, chitosan treatment modified H(+)-mediated 
      processes, in particular it inhibited the uptake of the H(+)-substrate 
      co-transported sucrose and valine, and inhibited the light-induced 
      H(+)/K(+)-mediated turgor reaction of motor cells. The present data also allowed 
      the limit of the cytotoxicity of the compound to be established close to a 
      concentration of 100 microg ml(-1) at the plasma membrane level. As a 
      consequence, chitosan could be preferably used in plant disease control as a 
      powerful elicitor rather than a direct antifungal agent.
FAU - Amborabe, Benigne-Ernest
AU  - Amborabe BE
AD  - University of Poitiers, Laboratoire de Biochimie, Physiologie et Biologie 
      Moleculaires Vegetales, FRE CNRS 3091, 40, Avenue du Recteur Pineau, F-86022 
      Poitiers cedex, France.
FAU - Bonmort, Janine
AU  - Bonmort J
FAU - Fleurat-Lessard, Pierrette
AU  - Fleurat-Lessard P
FAU - Roblin, Gabriel
AU  - Roblin G
LA  - eng
PT  - Journal Article
DEP - 20080531
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
RN  - 0 (Plant Proteins)
RN  - 9012-76-4 (Chitosan)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
SB  - IM
MH  - Biological Transport
MH  - Cell Membrane/*drug effects/enzymology/physiology
MH  - Cell Polarity
MH  - Chitosan/*pharmacology
MH  - Coated Vesicles/drug effects/physiology/ultrastructure
MH  - Electrophysiology
MH  - Hydrogen-Ion Concentration
MH  - Mimosa/*drug effects/enzymology/physiology
MH  - Plant Proteins/antagonists & inhibitors/metabolism
MH  - Proton-Translocating ATPases/antagonists & inhibitors/*metabolism
EDAT- 2008/06/03 09:00
MHDA- 2008/08/07 09:00
CRDT- 2008/06/03 09:00
PHST- 2008/06/03 09:00 [pubmed]
PHST- 2008/08/07 09:00 [medline]
PHST- 2008/06/03 09:00 [entrez]
AID - ern096 [pii]
AID - 10.1093/jxb/ern096 [doi]
PST - ppublish
SO  - J Exp Bot. 2008;59(9):2317-24. doi: 10.1093/jxb/ern096. Epub 2008 May 31.