PMID- 1851812
OWN - NLM
STAT- MEDLINE
DCOM- 19910621
LR  - 20081121
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 72 ( Pt 5)
DP  - 1991 May
TI  - Activation of gene expression by human herpesvirus 6 is reporter gene-dependent.
PG  - 1123-30
AB  - Infection with human herpesvirus 6 (HHV-6) was found to up-regulate expression of 
      human immunodeficiency virus and human T cell leukaemia virus type I (HTLV-I) 
      long terminal repeat sequence (LTR), and herpes simplex virus type 1 (HSV-1) gD 
      chloramphenicol acetyltransferase (CAT) constructs transfected into the T cell 
      line, J. Jhan. Activation by HHV-6 was due to one or more viral proteins produced 
      early in infection and, in the case of the HTLV-I LTR, was synergistic to 
      induction mediated by the HTLV-I tax gene product. Neither the HTLV-I enhancer 
      nor basal promoter elements of the HSV-1 gD gene were essential for activation 
      and no increase in accumulated HTLV-I mRNA was observed due to HHV-6 infection. 
      Induction by HHV-6 was found to be dependent on the reporter construct used, 
      because the CAT gene and, to a lesser extent, the HSV-1 thymidine kinase gene 
      were responsive to HHV-6 infection although no significant activation of growth 
      hormone constructs was observed. Our results bear a strong resemblance to those 
      obtained for the Epstein-Barr virus BMLF1 gene, indicating that the major HHV-6 
      trans-activator may be a homologue of this gene.
FAU - Campbell, M E
AU  - Campbell ME
AD  - Department of Veterinary Pathology, Glasgow University, U.K.
FAU - McCorkindale, S
AU  - McCorkindale S
FAU - Everett, R D
AU  - Everett RD
FAU - Onions, D E
AU  - Onions DE
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - Chloramphenicol O-Acetyltransferase/genetics
MH  - Chromosome Deletion
MH  - DNA, Viral/genetics
MH  - *Gene Expression Regulation, Viral
MH  - *Genes, Viral
MH  - Herpesvirus 6, Human/*genetics/physiology
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Mutation
MH  - Plasmids
MH  - Promoter Regions, Genetic
MH  - Transcription, Genetic
MH  - Transfection
MH  - Up-Regulation
MH  - Virus Replication
EDAT- 1991/05/01 00:00
MHDA- 1991/05/01 00:01
CRDT- 1991/05/01 00:00
PHST- 1991/05/01 00:00 [pubmed]
PHST- 1991/05/01 00:01 [medline]
PHST- 1991/05/01 00:00 [entrez]
AID - 10.1099/0022-1317-72-5-1123 [doi]
PST - ppublish
SO  - J Gen Virol. 1991 May;72 ( Pt 5):1123-30. doi: 10.1099/0022-1317-72-5-1123.