PMID- 18519810
OWN - NLM
STAT- MEDLINE
DCOM- 20080916
LR  - 20231105
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 112
IP  - 4
DP  - 2008 Aug 15
TI  - Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic 
      receptor DEC-205 stimulates protective T-cell responses.
PG  - 1231-9
LID - 10.1182/blood-2008-03-148072 [doi]
AB  - Dendritic cells (DCs) express many endocytic receptors that deliver antigens for 
      major histocompatibility class (MHC) I and II presentation to CD8(+) and CD4(+) T 
      cells, respectively. Here, we show that targeting Epstein-Barr virus (EBV) 
      nuclear antigen 1 (EBNA1) to one of them, the human multilectin DEC-205 receptor, 
      in the presence of the DC maturation stimulus poly(I:C), expanded EBNA1-specific 
      CD4(+) and CD8(+) memory T cells, and these lymphocytes could control the 
      outgrowth of autologous EBV-infected B cells in vitro. In addition, using a novel 
      mouse model with reconstituted human immune system components, we demonstrated 
      that vaccination with alphaDEC-205-EBNA1 antibodies primed EBNA1-specific 
      IFN-gamma-secreting T cells and also induced anti-EBNA1 antibodies in a subset of 
      immunized mice. Because EBNA1 is the one EBV antigen that is expressed in all 
      proliferating cells infected with this virus, our data suggest that DEC-205 
      targeting should be explored as a vaccination approach against symptomatic 
      primary EBV infection and against EBV-associated malignancies.
FAU - Gurer, Cagan
AU  - Gurer C
AD  - Laboratory of Viral Immunobiology, Christopher H Browne Center for Immunology and 
      Immune Diseases, The Rockefeller University, New York, NY 10065, USA.
FAU - Strowig, Till
AU  - Strowig T
FAU - Brilot, Fabienne
AU  - Brilot F
FAU - Pack, Maggi
AU  - Pack M
FAU - Trumpfheller, Christine
AU  - Trumpfheller C
FAU - Arrey, Frida
AU  - Arrey F
FAU - Park, Chae Gyu
AU  - Park CG
FAU - Steinman, Ralph M
AU  - Steinman RM
FAU - Munz, Christian
AU  - Munz C
LA  - eng
GR  - R01 CA108609/CA/NCI NIH HHS/United States
GR  - R01 CA108609-04A2/CA/NCI NIH HHS/United States
GR  - R01 CA101741/CA/NCI NIH HHS/United States
GR  - CA101741/CA/NCI NIH HHS/United States
GR  - R01CA101741/CA/NCI NIH HHS/United States
GR  - R01CA108609/CA/NCI NIH HHS/United States
GR  - CA108609/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080602
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, CD)
RN  - 0 (DEC-205 receptor)
RN  - 0 (Epstein-Barr Virus Nuclear Antigens)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral
MH  - Antigens, CD/*immunology
MH  - B-Lymphocytes/virology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - *Cell Proliferation
MH  - Dendritic Cells/immunology
MH  - Epstein-Barr Virus Nuclear Antigens/*immunology
MH  - Herpesvirus 4, Human/*immunology
MH  - Humans
MH  - Immunologic Memory
MH  - Lectins, C-Type/*immunology
MH  - *Lymphocyte Activation
MH  - Mice
MH  - Mice, Transgenic
MH  - Minor Histocompatibility Antigens
MH  - Receptors, Cell Surface/*immunology
MH  - T-Lymphocytes/*immunology
MH  - Vaccination
PMC - PMC2515117
EDAT- 2008/06/04 09:00
MHDA- 2008/09/17 09:00
PMCR- 2009/08/15
CRDT- 2008/06/04 09:00
PHST- 2008/06/04 09:00 [pubmed]
PHST- 2008/09/17 09:00 [medline]
PHST- 2008/06/04 09:00 [entrez]
PHST- 2009/08/15 00:00 [pmc-release]
AID - S0006-4971(20)49712-5 [pii]
AID - 2008/148072 [pii]
AID - 10.1182/blood-2008-03-148072 [doi]
PST - ppublish
SO  - Blood. 2008 Aug 15;112(4):1231-9. doi: 10.1182/blood-2008-03-148072. Epub 2008 
      Jun 2.