PMID- 18520085
OWN - NLM
STAT- MEDLINE
DCOM- 20080721
LR  - 20190706
IS  - 0009-2363 (Print)
IS  - 0009-2363 (Linking)
VI  - 56
IP  - 6
DP  - 2008 Jun
TI  - Identification and comparative determination of rhodionin in traditional tibetan 
      medicinal plants of fourteen Rhodiola species by high-performance liquid 
      chromatography-photodiode array detection and electrospray ionization-mass 
      spectrometry.
PG  - 807-14
AB  - Using the HPLC/PDA/ESI/MS method, a comparative analysis of rhodionin (RH) was 
      undertaken in order to conduct a qualitative and quantitative study in 38 batches 
      of fourteen species of Rhodiola for quality control purposes. Alongside of this 
      RH analysis, a simultaneous determination of salidroside (SA), tyrosol (TY), and 
      gallic acid (GA) was carried out. Rhodiola plants are a popularly used ethnodrug 
      from the Qinghai-Tibetan plateau of China. The identity of RH was unambiguously 
      determined based on the quasimolecular ions in negative ESI-MS mode. This method 
      was validated in respect to sensitivity, linearity, precision, repeatability and 
      recovery using optimized chromatographic conditions. The linear calibration curve 
      was acquired with R(2)>0.999, and the limit of detection (S/N=3) was estimated to 
      be 43.75 microg/g. The relative standard deviations (RSDs) of the intra- and 
      inter-day precisions were 0.75% and 0.50%, respectively. The repeatability was 
      evaluated by a replicated analysis of samples with the RSD value found within 
      0.67%. The recovery rates varied within the range of 98.79-100.08% with RSD less 
      than 1.10%. In the present study, the content of RH was quantified within 
      0.4192-4.7260 mg/g for 16 batches of R. crenulata. It was also found in eight 
      other species plants. The results demonstrated that RH is a useful characteristic 
      standard compound for quality evaluation and chemical differentiation among 
      species of Rhodiola. The study also indicated that the analytical procedure is 
      precise, reproducible and a potential tool for both quality assessment and 
      species identification.
FAU - Li, Tao
AU  - Li T
AD  - West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, PR China.
FAU - Zhang, Hao
AU  - Zhang H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Chem Pharm Bull (Tokyo)
JT  - Chemical & pharmaceutical bulletin
JID - 0377775
RN  - 0 (Flavonoids)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Monosaccharides)
RN  - 0 (Solutions)
RN  - 889FQ29409 (rhodionin)
SB  - IM
MH  - Calibration
MH  - Chromatography, High Pressure Liquid
MH  - Electrochemistry
MH  - Flavonoids/*chemistry
MH  - Indicators and Reagents
MH  - Monosaccharides/*chemistry
MH  - Online Systems
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Rhodiola/*chemistry
MH  - Solutions
MH  - Species Specificity
MH  - Spectrometry, Mass, Electrospray Ionization
EDAT- 2008/06/04 09:00
MHDA- 2008/07/22 09:00
CRDT- 2008/06/04 09:00
PHST- 2008/06/04 09:00 [pubmed]
PHST- 2008/07/22 09:00 [medline]
PHST- 2008/06/04 09:00 [entrez]
AID - JST.JSTAGE/cpb/56.807 [pii]
AID - 10.1248/cpb.56.807 [doi]
PST - ppublish
SO  - Chem Pharm Bull (Tokyo). 2008 Jun;56(6):807-14. doi: 10.1248/cpb.56.807.