PMID- 18521338
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1357-714X (Print)
IS  - 1369-1643 (Electronic)
IS  - 1357-714X (Linking)
VI  - 6
IP  - 3
DP  - 2002
TI  - Differential Expression of IRS-1 and IRS-2 in Uterine Leiomyosarcomas with 
      Distinct Oncogenic Phenotypes: Lack of Correlation with Downstream Signaling 
      Events.
PG  - 89-96
LID - 10.1080/1357714021000065387 [doi]
AB  - PURPOSE: Insulin receptor substrates (IRSs) are essential for insulin-induced 
      mitogenic effects on several cell types but they also are involved in cell 
      transformation.We investigated whether the differential constitutive expression 
      and potential distinct downstream signaling events of IRS-1 and IRS-2 might be 
      related to discrete tumourigenic phenotypes of three human uterine leiomyosarcoma 
      cell lines, one of which was specifically isolated for the present study. METHODS 
      AND RESULTS: SK-UT-1B egressed effectively from a gellyfied Matrigel matrix and 
      grew as did DMR cells in an anchorage-independent manner in agar and induced 
      rapidly growing tumours in nude mice. On the contrary, SK-LMS-1 cells did not 
      emigrate from Matrigel, neither grew in agar nor were they tumourigenic. IRS-2 
      was highly expressed in the more malignant cell lines, whereas IRS-1 was present 
      only in SK-LMS-1 cells. However, upon insulin stimulation both IRS- 1 and IRS-2 
      were tyrosine phosphorylated with a similar kinetic in the respective cell lines; 
      furthermore, after 1 min of insulin stimulation PI3-kinase associated with IRSs 
      and after 2 min Shc was phosphorylated and associated with Grb2 with minor 
      differences detectable among the various cell lines in the duration of 
      phosphorylation and/or in their association irrespective of whether IRS-1 or 
      IRS-2 were expressed. DISCUSSION: Our findings tend to exclude that the 
      malignancy displayed by uterine leiomyosarcomas might be directly linked to the 
      activation of distinct IRS-1- or IRS-2-dependent pathways.
FAU - Colombatti, Alfonso
AU  - Colombatti A
AD  - Divisione di Oncologia Sperimentale 2 Centro di Riferimento Oncologico-IRCCS 
      Aviano 33081 Italy.
FAU - Russo, Pietro
AU  - Russo P
FAU - Cervi, Marta
AU  - Cervi M
FAU - Bogetto, Laura
AU  - Bogetto L
FAU - Wassermann, Bruna
AU  - Wassermann B
FAU - Mainiero, Fabrizio
AU  - Mainiero F
FAU - Spessotto, Paola
AU  - Spessotto P
LA  - eng
PT  - Journal Article
PL  - Egypt
TA  - Sarcoma
JT  - Sarcoma
JID - 9709257
PMC - PMC2395487
EDAT- 2008/06/04 09:00
MHDA- 2008/06/04 09:01
PMCR- 2002/09/01
CRDT- 2008/06/04 09:00
PHST- 2008/06/04 09:00 [pubmed]
PHST- 2008/06/04 09:01 [medline]
PHST- 2008/06/04 09:00 [entrez]
PHST- 2002/09/01 00:00 [pmc-release]
AID - S1357714X02000269 [pii]
AID - 10.1080/1357714021000065387 [doi]
PST - ppublish
SO  - Sarcoma. 2002;6(3):89-96. doi: 10.1080/1357714021000065387.