PMID- 18522714
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 1
DP  - 2008 Jun 3
TI  - Chromosomal assignment of canine THADA gene to CFA 10q25.
PG  - 11
LID - 10.1186/1755-8166-1-11 [doi]
AB  - BACKGROUND: Chromosomal translocations affecting the chromosome 2p21 cluster in a 
      450 kb breakpoint region are frequently observed in human benign thyroid 
      adenomas. THADA (thyroid adenoma associated) was identified as the affected gene 
      within this breakpoint region. In contrast to man tumours of the thyroid gland of 
      dogs (Canis lupus familiaris) constitute mainly as follicular cell carcinomas, 
      with malignant thyroid tumours being more frequent than benign thyroid adenomas. 
      In order to elucidate if the THADA gene is also a target of chromosomal 
      rearrangements in thyroid adenomas of the dog we have physically mapped the 
      canine THADA gene to canine chromosome 10.A PCR was established to screen a 
      canine genome library for a BAC clone containing the gene sequence of canine 
      THADA. Further PCR reactions were done using the identified BAC clone as a 
      template in order to verify the corresponding PCR product by sequencing.Canine 
      whole blood was incubated with colcemid in order to arrest the cultured cells in 
      metaphases. The verified BAC DNA was digoxigenin labeled and used as a probe in 
      fluorescence in situ hybridization (FISH). Ten well spread metaphases were 
      examined indicating a signal on canine chromosome 10 on both chromatids. A 
      detailed fine mapping was performed indicating the canine THADA gene locus on the 
      q-arm of chromosome 10. RESULTS: The canine THADA gene locus was mapped on 
      chromosome 10q25. Our mapping results obtained in this study following the 
      previously described nomenclature for the canine karyotype. CONCLUSION: We 
      analysed whether the THADA gene locus is a hotspot of canine chromosomal 
      rearrangements in canine neoplastic lesions of the thyroid and in addition might 
      play a role as a candidate gene for a possible malignant transformation of canine 
      thyroid adenomas. Although the available cytogenetic data of canine thyroid 
      adenomas are still insufficient the chromosomal region to which the canine THADA 
      has been mapped seems to be no hotspot of chromosomal aberrations seen in canine 
      thyroid adenomas.
FAU - Soller, Jan T
AU  - Soller JT
AD  - Centre for Human Genetics, University of Bremen, Leobener Strasse ZHG, 28359 
      Bremen, Germany.
AD  - Small Animal Clinic and Research Cluster of Excellence "REBIRTH", University of 
      Veterinary Medicine Hanover, Bischofsholer Damm 15, 30173 Hannover, Germany.
FAU - Beuing, Claudia
AU  - Beuing C
AD  - Small Animal Clinic and Research Cluster of Excellence "REBIRTH", University of 
      Veterinary Medicine Hanover, Bischofsholer Damm 15, 30173 Hannover, Germany.
FAU - Murua Escobar, Hugo
AU  - Murua Escobar H
AD  - Centre for Human Genetics, University of Bremen, Leobener Strasse ZHG, 28359 
      Bremen, Germany.
AD  - Small Animal Clinic and Research Cluster of Excellence "REBIRTH", University of 
      Veterinary Medicine Hanover, Bischofsholer Damm 15, 30173 Hannover, Germany.
FAU - Winkler, Susanne
AU  - Winkler S
AD  - Centre for Human Genetics, University of Bremen, Leobener Strasse ZHG, 28359 
      Bremen, Germany.
FAU - Reimann-Berg, Nicola
AU  - Reimann-Berg N
AD  - Centre for Human Genetics, University of Bremen, Leobener Strasse ZHG, 28359 
      Bremen, Germany.
FAU - Drieschner, Norbert
AU  - Drieschner N
AD  - Centre for Human Genetics, University of Bremen, Leobener Strasse ZHG, 28359 
      Bremen, Germany.
FAU - Dolf, Gaudenz
AU  - Dolf G
AD  - Institute of Genetics, Vetsuisse Faculty, University of Berne, Bremgartenstrasse 
      109a, PO Box 8466, 3001 Bern, Switzerland.
FAU - Schelling, Claude
AU  - Schelling C
AD  - Department of Animal Science, Swiss Federal Institute of Technology Zurich, 
      Vetsuisse-Faculty Zurich, University of Zurich, Winterthurerstrasse 204, 8057 
      Zurich, Switzerland.
FAU - Nolte, Ingo
AU  - Nolte I
AD  - Small Animal Clinic and Research Cluster of Excellence "REBIRTH", University of 
      Veterinary Medicine Hanover, Bischofsholer Damm 15, 30173 Hannover, Germany.
FAU - Bullerdiek, Jorn
AU  - Bullerdiek J
AD  - Centre for Human Genetics, University of Bremen, Leobener Strasse ZHG, 28359 
      Bremen, Germany.
AD  - Small Animal Clinic and Research Cluster of Excellence "REBIRTH", University of 
      Veterinary Medicine Hanover, Bischofsholer Damm 15, 30173 Hannover, Germany.
LA  - eng
PT  - Journal Article
DEP - 20080603
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC2430699
EDAT- 2008/06/05 09:00
MHDA- 2008/06/05 09:01
PMCR- 2008/06/03
CRDT- 2008/06/05 09:00
PHST- 2008/03/13 00:00 [received]
PHST- 2008/06/03 00:00 [accepted]
PHST- 2008/06/05 09:00 [pubmed]
PHST- 2008/06/05 09:01 [medline]
PHST- 2008/06/05 09:00 [entrez]
PHST- 2008/06/03 00:00 [pmc-release]
AID - 1755-8166-1-11 [pii]
AID - 10.1186/1755-8166-1-11 [doi]
PST - epublish
SO  - Mol Cytogenet. 2008 Jun 3;1:11. doi: 10.1186/1755-8166-1-11.