PMID- 18522857
OWN - NLM
STAT- MEDLINE
DCOM- 20081006
LR  - 20080918
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 253
IP  - 1-2
DP  - 2008 May-Jun
TI  - Butyrate inhibits functional differentiation of human monocyte-derived dendritic 
      cells.
PG  - 54-8
LID - 10.1016/j.cellimm.2008.04.016 [doi]
AB  - Dendritic cells (DCs) play a key role in immune function through antigen 
      presentation by MHC and CD1, as well as cytokine production that shapes the 
      immune response. Here we report that butyrate, a histone deacetylase inhibitor, 
      inhibits the functional differentiation of human monocyte-derived DCs. Mature DCs 
      were generated from monocytes in the presence of granulocyte macrophage 
      colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4), followed by 2 day 
      LPS stimulation. Butyrate treatment throughout the culture period inhibited the 
      expression of CD1 molecules, but not on CD83, CD86, and MHC molecules. The 
      suppression was exerted at protein and mRNA levels. Butyrate-treated immature DCs 
      also showed decreased expression of CD1 molecules. Moreover the butyrate-treated 
      immature DCs showed lower production of IL-12 p40 and IL-6 in response to 
      lipopolysaccharides and induced less Th1 cells in allogenic mixed lymphocyte 
      reactions. Our results imply that histone acetylation is involved in regulating 
      immune responses through regulating functional differentiation of DC. Thus HDAC 
      may be one of the targets for controlling the immune response.
FAU - Wang, Biao
AU  - Wang B
AD  - Department of Laboratory Medicine and Evidence-based Laboratory Medicine, Kobe 
      University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 
      650-0017, Japan.
FAU - Morinobu, Akio
AU  - Morinobu A
FAU - Horiuchi, Marika
AU  - Horiuchi M
FAU - Liu, Jun
AU  - Liu J
FAU - Kumagai, Shunichi
AU  - Kumagai S
LA  - eng
PT  - Journal Article
DEP - 20080605
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Antigens, CD)
RN  - 0 (Butyrates)
RN  - 0 (Cytokines)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Antigens, CD/metabolism
MH  - Butyrates/*pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/cytology/*physiology
MH  - HLA Antigens/metabolism
MH  - Humans
MH  - Monocytes/cytology/*physiology
MH  - Phenotype
MH  - Th1 Cells/cytology/immunology
MH  - Th2 Cells/cytology/immunology
EDAT- 2008/06/05 09:00
MHDA- 2008/10/07 09:00
CRDT- 2008/06/05 09:00
PHST- 2008/01/04 00:00 [received]
PHST- 2008/04/07 00:00 [revised]
PHST- 2008/04/23 00:00 [accepted]
PHST- 2008/06/05 09:00 [pubmed]
PHST- 2008/10/07 09:00 [medline]
PHST- 2008/06/05 09:00 [entrez]
AID - S0008-8749(08)00082-8 [pii]
AID - 10.1016/j.cellimm.2008.04.016 [doi]
PST - ppublish
SO  - Cell Immunol. 2008 May-Jun;253(1-2):54-8. doi: 10.1016/j.cellimm.2008.04.016. 
      Epub 2008 Jun 5.