PMID- 18523354
OWN - NLM
STAT- MEDLINE
DCOM- 20090211
LR  - 20080604
IS  - 1735-1383 (Print)
IS  - 1735-1383 (Linking)
VI  - 5
IP  - 2
DP  - 2008 Jun
TI  - HLA-DRB1, DQA1 and DQB1 alleles and haplotypes frequencies in Iranian healthy 
      adult responders and non-responders to recombinant hepatitis B vaccine.
PG  - 92-9
AB  - BACKGROUND: Different studies have demonstrated that a small proportion of 
      healthy individuals receiving the hepatitis B (HB) vaccine do not produce 
      protective levels of anti-HB antibody, a phenomenon which could be linked to 
      certain human leukocyte antigen (HLA) class-II alleles or haplotypes. OBJECTIVES: 
      The present study was undertaken to determine the frequency of HLA class-II 
      alleles in Iranian healthy adult responders and non-responders to HB vaccine. 
      METHODS: Twelve non-responders (anti-HBs antibody<10 IU/L) and 46 responders 
      (anti-HBs antibody>100 IU/L) were tissue typed for HLA class-II. HLA-DRB1, DQB1 
      and DQA1 alleles were determined using polymerase chain reaction based on 
      sequence specific primers (PCR-SSP) technique. Accessibility to excess amount of 
      genomic DNA was possible using Epstein-Barr virus (EBV)-transformed B-cells 
      established from all vaccinees. RESULTS: Our results demonstrated increased 
      frequencies of HLA- DRB107, DRB103, DRB104, DQB10201, DQA10201 alleles and HLA- 
      DRB107/DQB10201/DQA10201 and DRB104/DQB10302/DQA103011 haplotypes in the 
      non-responder group. Comparison between responders and non-responders revealed 
      only a significant difference for DQB10201 allele (p<0.05). CONCLUSION: These 
      findings confirm the association of certain HLA alleles and haplotypes with the 
      lack of antibody response to HB vaccine in an Iranian population.
FAU - Amirzargar, Ali Akbar
AU  - Amirzargar AA
AD  - National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
FAU - Mohseni, Nilufar
AU  - Mohseni N
FAU - Shokrgozar, Mohammad Ali
AU  - Shokrgozar MA
FAU - Arjang, Zohreh
AU  - Arjang Z
FAU - Ahmadi, Nahid
AU  - Ahmadi N
FAU - Yousefi Behzadi, Manijeh
AU  - Yousefi Behzadi M
FAU - Amanzadeh, Amir
AU  - Amanzadeh A
FAU - Shokri, Fazel
AU  - Shokri F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Iran
TA  - Iran J Immunol
JT  - Iranian journal of immunology : IJI
JID - 101282932
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Hepatitis B Vaccines)
RN  - 0 (Vaccines, Synthetic)
SB  - IM
MH  - Adult
MH  - *Alleles
MH  - Female
MH  - HLA-DQ Antigens/genetics/*immunology
MH  - HLA-DR Antigens/genetics/*immunology
MH  - Haplotypes/genetics/*immunology
MH  - Health
MH  - Hepatitis B Vaccines/genetics/*immunology
MH  - Humans
MH  - Male
MH  - Vaccines, Synthetic/genetics/*immunology
EDAT- 2008/06/05 09:00
MHDA- 2009/02/12 09:00
CRDT- 2008/06/05 09:00
PHST- 2008/06/05 09:00 [pubmed]
PHST- 2009/02/12 09:00 [medline]
PHST- 2008/06/05 09:00 [entrez]
AID - 03 [pii]
PST - ppublish
SO  - Iran J Immunol. 2008 Jun;5(2):92-9.