PMID- 18524543
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20171116
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 52
IP  - 1
DP  - 2008 Oct
TI  - Regulatory effects of antihistamines on the responses to staphylococcal 
      enterotoxin B of human monocyte-derived dendritic cells and CD4+ T cells.
PG  - 31-8
LID - 10.1016/j.jdermsci.2008.04.004 [doi]
AB  - BACKGROUND: Antihistamines are widely used for the treatment of allergic 
      diseases, such as urticaria and allergic rhinitis. They are also effective for 
      the treatment of diseases in which T cells are mainly involved in the 
      pathogenesis, such as atopic dermatitis (AD) and contact dermatitis. Dendritic 
      cells (DCs) drive polarization of naive T cells into Th1 or Th2 subsets, and are 
      also likely to be involved in AD pathogenesis. OBJECTIVES: The aim of this study 
      was to determine the effects of antihistamines on DCs and CD4+ T cells. METHODS: 
      Human monocyte-derived DCs (MoDCs) and autologous CD4+ T cells were obtained from 
      healthy subjects, and cultured together or independently in the presence of 
      antihistamines. As a stimulant, we used staphylococcal enterotoxin B or the 
      combination of anti-CD3 monoclonal antibody (mAb) and anti-CD28 mAb. The 
      concentrations of cytokines and chemokines in culture supernatants were measured 
      by ELISA. The expression of surface molecules on MoDCs was measured by flow 
      cytometry. Cell proliferation in the cocultures of MoDCs and CD4+ T cells (DC-T 
      cocultures) was measured by a [(3)H] thymidine incorporation assay. RESULTS: 
      Antihistamines inhibited the production of IFN-gamma, and enhanced the production 
      of IL-4 in DC-T cocultures. Antihistamines inhibited the production of TNF-alpha, 
      TARC, MDC, IP-10, and Mig from MoDCs. Epinastine, one of antihistamines, 
      suppressed the expression of ICAM-1 (CD54) on MoDCs. Epinastine also inhibited 
      the proliferation of CD4+ T cells cocultured with MoDCs. CONCLUSIONS: Our 
      findings show that antihistamines regulate immune responses by affecting the 
      interaction between DCs and CD4+ T cells, and further DCs and CD4+ T cells 
      independently, which may partially contribute to the control of allergic diseases 
      such as AD and contact dermatitis.
FAU - Iida, Hideyuki
AU  - Iida H
AD  - Department of Dermatology, Nara Medical University, Kashihara, Japan. 
      hidemaki@naramed-u.ac.jp
FAU - Asada, Hideo
AU  - Asada H
FAU - Yokoi, Shoko
AU  - Yokoi S
FAU - Niizeki, Hironori
AU  - Niizeki H
FAU - Yasuda, Yasuki
AU  - Yasuda Y
FAU - Miyagawa, Sachiko
AU  - Miyagawa S
FAU - Kita, Eiji
AU  - Kita E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080603
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CCL17 protein, human)
RN  - 0 (CD28 Antigens)
RN  - 0 (CD3 Complex)
RN  - 0 (CXCL10 protein, human)
RN  - 0 (CXCL9 protein, human)
RN  - 0 (Chemokine CCL17)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL9)
RN  - 0 (Enterotoxins)
RN  - 0 (Histamine H1 Antagonists)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 207137-56-2 (Interleukin-4)
RN  - 39424-53-8 (enterotoxin B, staphylococcal)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (ADAM11 protein, human)
SB  - IM
MH  - ADAM Proteins/antagonists & inhibitors/biosynthesis/immunology
MH  - Adult
MH  - Antibodies, Monoclonal/immunology
MH  - CD28 Antigens/immunology/metabolism
MH  - CD3 Complex/immunology/metabolism
MH  - CD4-Positive T-Lymphocytes/drug effects/*immunology/metabolism
MH  - Cell Proliferation/drug effects
MH  - Chemokine CCL17/biosynthesis
MH  - Chemokine CXCL10/biosynthesis
MH  - Chemokine CXCL9/biosynthesis
MH  - Coculture Techniques
MH  - Dendritic Cells/drug effects/*immunology/metabolism
MH  - Enterotoxins/*immunology
MH  - Histamine H1 Antagonists/*pharmacology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/immunology
MH  - Interferon-gamma/biosynthesis/*immunology
MH  - Interleukin-4/biosynthesis/*immunology
MH  - Middle Aged
MH  - Tumor Necrosis Factor-alpha/biosynthesis
MH  - Tumor Suppressor Proteins/antagonists & inhibitors/biosynthesis/immunology
EDAT- 2008/06/06 09:00
MHDA- 2008/11/19 09:00
CRDT- 2008/06/06 09:00
PHST- 2007/07/11 00:00 [received]
PHST- 2008/03/10 00:00 [revised]
PHST- 2008/04/07 00:00 [accepted]
PHST- 2008/06/06 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2008/06/06 09:00 [entrez]
AID - S0923-1811(08)00122-9 [pii]
AID - 10.1016/j.jdermsci.2008.04.004 [doi]
PST - ppublish
SO  - J Dermatol Sci. 2008 Oct;52(1):31-8. doi: 10.1016/j.jdermsci.2008.04.004. Epub 
      2008 Jun 3.