PMID- 18535836 OWN - NLM STAT- MEDLINE DCOM- 20080821 LR - 20211020 IS - 1439-6319 (Print) IS - 1439-6319 (Linking) VI - 104 IP - 1 DP - 2008 Sep TI - Decrease in Akt/PKB signalling in human skeletal muscle by resistance exercise. PG - 57-65 LID - 10.1007/s00421-008-0786-7 [doi] AB - We analysed the effects of resistance exercise upon the phosphorylation state of proteins associated with adaptive processes from the Akt/PKB (protein kinase B) and the mitogen-activated protein kinase (MAPK) pathways. Nine healthy young men (21.7 +/- 0.55 year) performed 10 sets of 10 leg extensions at 80% of their 1-RM (repetition maximum). Muscle biopsies were taken from the vastus lateralis at rest, within the first 30 s after exercise and at 24 h post-exercise. Immediately post exercise, the phosphorylation states of Akt/PKB on Thr308 and Ser473 and 4E-BP1 on Thr37/46 (eukaryotic initiation factor 4E-binding protein 1) were decreased (-60 to -90%, P < 0.05). Conversely, the phosphorylation of p70(s6k) (p70 ribosomal S6 kinase) on Thr421/Ser424 was increased more than 20-fold (P < 0.05), and this was associated with a 10- to 50-fold increase in the phosphorylation of p38 and ERK1/2 (extracellular signal-regulated kinase) (P < 0.05). Twenty-four hours post-exercise the phosphorylation state of Akt/PKB on Thr308 was depressed, whereas the phosphorylation of p70(s6k) on Thr421/Ser424 and sarcoplasmic ERK1/2 were elevated. The present results indicate that high-intensity resistance exercise in the fasted state inhibits Akt/PKB and 4E-BP1 whilst concomitantly augmenting MAPK signalling and p70(s6k) on Thr421/Ser424. FAU - Deldicque, Louise AU - Deldicque L AD - Department of Physical Education and Rehabilitation, Universite catholique de Louvain, Place Pierre de Coubertin 1, Louvain-la-Neuve, Belgium. FAU - Atherton, Philip AU - Atherton P FAU - Patel, Rekha AU - Patel R FAU - Theisen, Daniel AU - Theisen D FAU - Nielens, Henri AU - Nielens H FAU - Rennie, Michael J AU - Rennie MJ FAU - Francaux, Marc AU - Francaux M LA - eng GR - AR 49869/AR/NIAMS NIH HHS/United States GR - BB/C516779/1/Biotechnology and Biological Sciences Research Council/United Kingdom GR - BB/X510697/1/Biotechnology and Biological Sciences Research Council/United Kingdom PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20080606 PL - Germany TA - Eur J Appl Physiol JT - European journal of applied physiology JID - 100954790 RN - 0 (Adaptor Proteins, Signal Transducing) RN - 0 (Cell Cycle Proteins) RN - 0 (EIF4EBP1 protein, human) RN - 0 (Phosphoproteins) RN - 0 (Protein Kinase Inhibitors) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) RN - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa) RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1) RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3) RN - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) SB - IM MH - Adaptor Proteins, Signal Transducing/metabolism MH - Adult MH - Animals MH - Cell Cycle Proteins MH - Cell Line MH - Exercise/*physiology MH - Fasting/metabolism MH - Humans MH - Male MH - Mice MH - Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/metabolism MH - Mitogen-Activated Protein Kinase 3/antagonists & inhibitors/metabolism MH - *Muscle Contraction MH - Myoblasts, Skeletal/drug effects/metabolism MH - Phosphoproteins/metabolism MH - Phosphorylation MH - Protein Kinase Inhibitors/pharmacology MH - Proto-Oncogene Proteins c-akt/*metabolism MH - Quadriceps Muscle/enzymology/*metabolism MH - Ribosomal Protein S6 Kinases, 70-kDa/metabolism MH - *Signal Transduction/drug effects MH - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism EDAT- 2008/06/07 09:00 MHDA- 2008/08/22 09:00 CRDT- 2008/06/07 09:00 PHST- 2008/05/26 00:00 [accepted] PHST- 2008/06/07 09:00 [pubmed] PHST- 2008/08/22 09:00 [medline] PHST- 2008/06/07 09:00 [entrez] AID - 10.1007/s00421-008-0786-7 [doi] PST - ppublish SO - Eur J Appl Physiol. 2008 Sep;104(1):57-65. doi: 10.1007/s00421-008-0786-7. Epub 2008 Jun 6.