PMID- 18538373
OWN - NLM
STAT- MEDLINE
DCOM- 20080930
LR  - 20211020
IS  - 0047-6374 (Print)
IS  - 0047-6374 (Linking)
VI  - 129
IP  - 9
DP  - 2008 Sep
TI  - Age-related decrease in expression of mitochondrial DNA encoded subunits of 
      cytochrome c oxidase in Drosophila melanogaster.
PG  - 558-61
LID - 10.1016/j.mad.2008.04.006 [doi]
AB  - A key feature of the aging process is that the mitochondrial respiratory capacity 
      declines and, the production of reactive oxygen species increases in the later 
      part of life span. In previous studies, cytochrome c oxidase (CcO), the terminal 
      component of the mitochondrial electron transport chain, was found to be the only 
      oxidoreductase exhibiting an age-related decrease in activity in Drosophila 
      melanogaster. The present study tested the hypothesis that decreases in the 
      abundance of catalytic subunits of CcO, encoded in mitochondrial DNA, could 
      underlie the age-associated loss of enzyme activity. Protein amounts of subunits 
      I, II and III, which form the catalytic core of CcO, were determined by 
      immunoblot analysis in 15-, 25-, 35-, 47- and 60-day-old flies. Subunits II and 
      III decreased with age by up to 43% and 75%, respectively, whereas the decrease 
      in subunit I was only 15%. The results pinpoint specific changes in a component 
      of the mitochondrial electron transport chain, which could underlie the 
      age-related decrease in mitochondrial respiratory activity and an increase in 
      oxidant production. Apparently, the stoichiometry of CcO holoprotein is 
      dynamically altered during the aging process in D. melanogaster.
FAU - Sohal, Rajindar S
AU  - Sohal RS
AD  - Department of Pharmacology and Pharmaceutical Sciences, University of Southern 
      California, 1985 Zonal Avenue, Los Angeles, CA 90033, United States. 
      sohal@usc.edu
FAU - Toroser, Dikran
AU  - Toroser D
FAU - Bregere, Catherine
AU  - Bregere C
FAU - Mockett, Robin J
AU  - Mockett RJ
FAU - Orr, William C
AU  - Orr WC
LA  - eng
GR  - R01 AG007657/AG/NIA NIH HHS/United States
GR  - R01 AG007657-17/AG/NIA NIH HHS/United States
GR  - R01 AG7657/AG/NIA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080422
PL  - Ireland
TA  - Mech Ageing Dev
JT  - Mechanisms of ageing and development
JID - 0347227
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (Protein Subunits)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Aging/*genetics/metabolism
MH  - Animals
MH  - Cattle
MH  - DNA, Mitochondrial/*physiology
MH  - Drosophila melanogaster/*enzymology/genetics
MH  - Electron Transport Complex IV/biosynthesis/*genetics
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Gene Expression Regulation, Enzymologic/*physiology
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mitochondria/*enzymology/genetics
MH  - Protein Subunits/biosynthesis/*genetics
MH  - Rats
PMC - PMC2519959
MID - NIHMS60745
EDAT- 2008/06/10 09:00
MHDA- 2008/10/01 09:00
PMCR- 2009/09/01
CRDT- 2008/06/10 09:00
PHST- 2008/02/08 00:00 [received]
PHST- 2008/04/08 00:00 [revised]
PHST- 2008/04/12 00:00 [accepted]
PHST- 2008/06/10 09:00 [pubmed]
PHST- 2008/10/01 09:00 [medline]
PHST- 2008/06/10 09:00 [entrez]
PHST- 2009/09/01 00:00 [pmc-release]
AID - S0047-6374(08)00098-5 [pii]
AID - 10.1016/j.mad.2008.04.006 [doi]
PST - ppublish
SO  - Mech Ageing Dev. 2008 Sep;129(9):558-61. doi: 10.1016/j.mad.2008.04.006. Epub 
      2008 Apr 22.