PMID- 18538757
OWN - NLM
STAT- MEDLINE
DCOM- 20081031
LR  - 20161124
IS  - 1090-2392 (Electronic)
IS  - 0011-2240 (Linking)
VI  - 57
IP  - 1
DP  - 2008 Aug
TI  - Prevention of cold ischemia/rewarming-induced ERK 1/2, p38 kinase and HO-1 
      activation by trophic factor supplementation of UW solution.
PG  - 72-4
LID - 10.1016/j.cryobiol.2008.04.003 [doi]
AB  - We have previously shown that trophic factor supplementation (TFS) of University 
      of Wisconsin (UW) solution reduced early apoptotic changes in vascular 
      endothelial cells. Here, we examine the effect of TFS on cell signaling pathways 
      related to cell growth, differentiation, and apoptosis after cold ischemic 
      storage. In this study, the effect of TFS on the phosphorylation of signaling 
      molecules ERK (extracellular regulated-signaling kinase) 1/2 and p38 MAPK 
      (mitogen activated protein kinases) and of HO-1 (hemeoxygenase-1), relative to 
      changes seen in unmodified UW solution, were determined by Western blot in cells 
      stored under cold ischemic conditions. Primary cultures of canine kidney proximal 
      tubule cells (CKPTC) and human umbilical vein endothelial cells (HUVEC) were used 
      in this study. There was a significant decrease, relative to UW solution, after 1 
      min rewarming in ERK 1 and 2 activity in CKPTCs. For p38 MAPK, a significant 
      decrease after 5 min rewarming was seen in CKPTC (p<0.05) while significant 
      reductions relative to UW solution were seen in HUVECs after both 1 and 5 min 
      rewarming (p<0.05). Phosphorylated HO-1 was also decreased by 43% and 50% in 
      HUVECs, relative to UW solution, after 1 and 5 min rewarming (p<0.05 at each time 
      point). Collectively, TFS not only limits ERK 1/2 and p38 MAPK activity induced 
      by cold ischemic injury and subsequent rewarming, but also substantially 
      restricted increases in HO-1 phosphorylation.
FAU - Kwon, Young Sam
AU  - Kwon YS
AD  - Department of Surgical Sciences, School of Veterinary Medicine, University of 
      Wisconsin, 2015 Linden Drive W, Madison, WI 53706-1102, USA.
FAU - Foley, John D
AU  - Foley JD
FAU - Russell, Paul
AU  - Russell P
FAU - McAnulty, Jonathan F
AU  - McAnulty JF
FAU - Murphy, Christopher J
AU  - Murphy CJ
LA  - eng
PT  - Journal Article
DEP - 20080425
PL  - Netherlands
TA  - Cryobiology
JT  - Cryobiology
JID - 0006252
RN  - 0 (Insulin)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Organ Preservation Solutions)
RN  - 0 (University of Wisconsin-lactobionate solution)
RN  - 63CZ7GJN5I (Allopurinol)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - GAN16C9B8O (Glutathione)
RN  - K72T3FS567 (Adenosine)
RN  - N5O3QU595M (Raffinose)
SB  - IM
MH  - Adenosine/metabolism/pharmacology
MH  - Allopurinol/metabolism/pharmacology
MH  - Animals
MH  - Apoptosis
MH  - Cells, Cultured
MH  - *Cold Ischemia
MH  - Dogs
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Glutathione/metabolism/pharmacology
MH  - Heme Oxygenase-1/*metabolism
MH  - Humans
MH  - Insulin/metabolism/pharmacology
MH  - Intercellular Signaling Peptides and Proteins/pharmacology
MH  - Kidney Tubules/cytology/drug effects
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Organ Preservation Solutions/metabolism/*pharmacology
MH  - Phosphorylation
MH  - Raffinose/metabolism/pharmacology
MH  - Signal Transduction
MH  - Wisconsin
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2008/06/10 09:00
MHDA- 2008/11/01 09:00
CRDT- 2008/06/10 09:00
PHST- 2007/11/24 00:00 [received]
PHST- 2008/04/05 00:00 [revised]
PHST- 2008/04/07 00:00 [accepted]
PHST- 2008/06/10 09:00 [pubmed]
PHST- 2008/11/01 09:00 [medline]
PHST- 2008/06/10 09:00 [entrez]
AID - S0011-2240(08)00053-9 [pii]
AID - 10.1016/j.cryobiol.2008.04.003 [doi]
PST - ppublish
SO  - Cryobiology. 2008 Aug;57(1):72-4. doi: 10.1016/j.cryobiol.2008.04.003. Epub 2008 
      Apr 25.