PMID- 18538899
OWN - NLM
STAT- MEDLINE
DCOM- 20080911
LR  - 20151119
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 26
IP  - 29-30
DP  - 2008 Jul 4
TI  - Development of a porcine reproductive and respiratory syndrome virus 
      differentiable (DIVA) strain through deletion of specific immunodominant 
      epitopes.
PG  - 3594-600
LID - 10.1016/j.vaccine.2008.04.078 [doi]
AB  - The availability of a DIVA (differentiating infected from vaccinated animals) 
      vaccine is very important for the control and eradication of endemic infectious 
      diseases such as porcine reproductive and respiratory syndrome (PRRS). Previous 
      studies in our laboratory identified several B-cell linear epitopes consistently 
      recognized by convalescent sera obtained from pigs infected with a North American 
      porcine reproductive and respiratory syndrome virus (PRRSV) strain. To ascertain 
      if one or more of these immunodominant epitopes can be used as the basis of DIVA 
      differentiation, we selected two epitope markers previously identified on the 
      non-structural protein 2 (PRRSV NSP2, predictably the viral protein most likely 
      to tolerate large deletions). The choice of these epitopes was primarily based on 
      their immunodominance and their deletion were performed along the backbone of the 
      wild-type cDNA infectious clone (FL12). We were able to successfully rescue a 
      mutant that fulfilled the requirements for a DIVA marker strain, such as: 
      efficient growth of the deletion mutant in vitro and in vivo and induction of 
      specific seroconversion as measured by a commercial ELISA kit, with absence of a 
      marker-specific peptide-ELISA response in 100% (n=15) of the inoculated animals. 
      In summary, our results provide proof of concept that DIVA PRRSV vaccines can 
      potentially be developed by deletion of individual "marker" immunodominant 
      epitopes.
FAU - de Lima, Marcelo
AU  - de Lima M
AD  - Nebraska Center for Virology and Department of Veterinary and Biomedical 
      Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583-0900, USA.
FAU - Kwon, Byungjoon
AU  - Kwon B
FAU - Ansari, Israrul H
AU  - Ansari IH
FAU - Pattnaik, Asit K
AU  - Pattnaik AK
FAU - Flores, Eduardo F
AU  - Flores EF
FAU - Osorio, Fernando A
AU  - Osorio FA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080519
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Viral)
RN  - 0 (Biomarkers)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Biomarkers
MH  - Immunodominant Epitopes/*genetics/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/growth & 
      development/*immunology
MH  - Sequence Deletion
MH  - Swine
MH  - Viral Vaccines/genetics/immunology
MH  - Viremia
EDAT- 2008/06/10 09:00
MHDA- 2008/09/13 09:00
CRDT- 2008/06/10 09:00
PHST- 2008/03/14 00:00 [received]
PHST- 2008/04/24 00:00 [revised]
PHST- 2008/04/30 00:00 [accepted]
PHST- 2008/06/10 09:00 [pubmed]
PHST- 2008/09/13 09:00 [medline]
PHST- 2008/06/10 09:00 [entrez]
AID - S0264-410X(08)00554-9 [pii]
AID - 10.1016/j.vaccine.2008.04.078 [doi]
PST - ppublish
SO  - Vaccine. 2008 Jul 4;26(29-30):3594-600. doi: 10.1016/j.vaccine.2008.04.078. Epub 
      2008 May 19.