PMID- 18539059
OWN - NLM
STAT- MEDLINE
DCOM- 20090728
LR  - 20090525
IS  - 1090-0233 (Print)
IS  - 1090-0233 (Linking)
VI  - 181
IP  - 3
DP  - 2009 Sep
TI  - In vitro culture of equine respiratory mucosa explants.
PG  - 280-7
LID - 10.1016/j.tvjl.2008.03.027 [doi]
AB  - An in vitro model of the upper respiratory tract of the horse was developed to 
      investigate mechanisms of respiratory diseases. Four tissues of the upper 
      respiratory tract of three horses were collected. Explants were maintained in 
      culture at an air-liquid interface for 96h. At 0, 24, 48, 72 and 96h of 
      cultivation, a morphometric analysis was performed using light microscopy, 
      scanning electron microscopy and transmission electron microscopy. The explants 
      were judged on morphometric changes of epithelium, basement membrane and 
      connective tissue. Viability was evaluated using a fluorescent Terminal 
      deoxynucleotidyl transferase-mediated dUTP Nick End Labelling (TUNEL) staining. 
      No significant changes in morphometry and viability of any of the explants were 
      observed during cultivation. Hence, the in vitro model may be useful to study 
      infectious and non-infectious diseases at the level of the equine respiratory 
      tract, with potential application to the development of vaccines and treatments 
      for diseases of the respiratory tract.
FAU - Vandekerckhove, Annelies
AU  - Vandekerckhove A
AD  - Department of Virology, Parasitology and Immunology, Faculty of Veterinary 
      Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
FAU - Glorieux, Sarah
AU  - Glorieux S
FAU - Broeck, Wim Van den
AU  - Broeck WV
FAU - Gryspeerdt, Annick
AU  - Gryspeerdt A
FAU - van der Meulen, Karen M
AU  - van der Meulen KM
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080606
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
SB  - IM
MH  - Animals
MH  - Cilia
MH  - Horses/*physiology
MH  - Microscopy
MH  - Nasopharynx
MH  - Palatine Tonsil/*anatomy & histology/*physiology
MH  - Respiratory Mucosa/*anatomy & histology/*physiology
MH  - Time Factors
MH  - Tissue Culture Techniques/*veterinary
MH  - Trachea
EDAT- 2008/06/10 09:00
MHDA- 2009/07/29 09:00
CRDT- 2008/06/10 09:00
PHST- 2007/12/21 00:00 [received]
PHST- 2008/03/13 00:00 [revised]
PHST- 2008/03/24 00:00 [accepted]
PHST- 2008/06/10 09:00 [pubmed]
PHST- 2009/07/29 09:00 [medline]
PHST- 2008/06/10 09:00 [entrez]
AID - S1090-0233(08)00138-X [pii]
AID - 10.1016/j.tvjl.2008.03.027 [doi]
PST - ppublish
SO  - Vet J. 2009 Sep;181(3):280-7. doi: 10.1016/j.tvjl.2008.03.027. Epub 2008 Jun 6.