PMID- 18541227 OWN - NLM STAT- MEDLINE DCOM- 20080915 LR - 20080701 IS - 0009-2797 (Print) IS - 0009-2797 (Linking) VI - 174 IP - 1 DP - 2008 Jul 10 TI - Effect of panaxydol on hypoxia-induced cell death and expression and secretion of neurotrophic factors (NTFs) in hypoxic primary cultured Schwann cells. PG - 44-50 LID - 10.1016/j.cbi.2008.04.041 [doi] AB - It has been shown that panaxydol (PND) can mimic the neurotrophic effect of nerve growth factor (NGF) normally secreted by Schwann cells (SC) and protect neurons against injury. To evaluate the effect of PND on hypoxia-induced SC death and expression and secretion of neurotrophic factors (NGF and brain derived neurotrophic factor (BDNF)), hypoxic SCs were cultured in vitro and then treated with PND (0-20 microM). The MTT (3(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) assay, immunocytochemistry, ELISA and RT-PCR were employed to examine the effects. We found that hypoxia resulted in a significant decrease in SCs viability (MTT: 64+/-4.7% of control group) and nearly a 3.3-fold increase of intracellular level of active caspase-3. PND (5-20 microM) treatment significantly rescued the SCs from hypoxia-induced injury (85+/-8.2%; 92+/-8.6%; 87+/-7.3%) and reduced caspase-3 activity with the maximal effect occurred at 10 microM (P<0.01), reducing to about 1.6-fold of control level. Furthermore, PND treatment also enhanced NGF and BDNF mRNA levels in hypoxic SCs and promoted protein expression and secretion. BDNF mRNA in hypoxic SCs was restored to about 90% of normal level and NGF mRNA was elevated to 1.4-fold of control after 10 microM PND treatment. These observations showed that PND protects primary cultured SCs against hypoxia-induced injury and enhances NTF-associated activities. FAU - Zhu, Hao AU - Zhu H AD - Department of Anatomy, Shanghai Jiao Tong University School of Medicine, No. 227 South Chongqing Road, Shanghai 200025, China. FAU - Wang, Wen-Jin AU - Wang WJ FAU - Ding, Wen-Long AU - Ding WL FAU - Li, Feng AU - Li F FAU - He, Jing AU - He J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080504 PL - Ireland TA - Chem Biol Interact JT - Chemico-biological interactions JID - 0227276 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Diynes) RN - 0 (Fatty Alcohols) RN - 0 (Nerve Growth Factors) RN - 0 (RNA, Messenger) RN - 72800-72-7 (panaxydol) RN - EC 3.4.22.- (Caspase 3) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Caspase 3/metabolism MH - Cell Death/drug effects MH - Cell Hypoxia/drug effects MH - Cell Survival/drug effects MH - Cells, Cultured MH - Diynes/*pharmacology MH - Enzyme Activation/drug effects MH - Fatty Alcohols/*pharmacology MH - Gene Expression Regulation/drug effects MH - Immunohistochemistry MH - Nerve Growth Factors MH - RNA, Messenger/genetics/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Schwann Cells/*cytology/drug effects/enzymology/*metabolism EDAT- 2008/06/11 09:00 MHDA- 2008/09/16 09:00 CRDT- 2008/06/11 09:00 PHST- 2007/12/24 00:00 [received] PHST- 2008/04/24 00:00 [revised] PHST- 2008/04/24 00:00 [accepted] PHST- 2008/06/11 09:00 [pubmed] PHST- 2008/09/16 09:00 [medline] PHST- 2008/06/11 09:00 [entrez] AID - S0009-2797(08)00244-5 [pii] AID - 10.1016/j.cbi.2008.04.041 [doi] PST - ppublish SO - Chem Biol Interact. 2008 Jul 10;174(1):44-50. doi: 10.1016/j.cbi.2008.04.041. Epub 2008 May 4.