PMID- 18541668
OWN - NLM
STAT- MEDLINE
DCOM- 20080825
LR  - 20220224
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 28
IP  - 16
DP  - 2008 Aug
TI  - Coa2 is an assembly factor for yeast cytochrome c oxidase biogenesis that 
      facilitates the maturation of Cox1.
PG  - 4927-39
LID - 10.1128/MCB.00057-08 [doi]
AB  - The assembly of cytochrome c oxidase (CcO) in yeast mitochondria is dependent on 
      a new assembly factor designated Coa2. Coa2 was identified from its ability to 
      suppress the respiratory deficiency of coa1Delta and shy1Delta cells. Coa1 and 
      Shy1 function at an early step in maturation of the Cox1 subunit of CcO. Coa2 
      functions downstream of the Mss51-Coa1 step in Cox1 maturation and likely 
      concurrent with the Shy1-related heme a(3) insertion into Cox1. Coa2 interacts 
      with Shy1. Cells lacking Coa2 show a rapid degradation of newly synthesized Cox1. 
      Rapid Cox1 proteolysis also occurs in shy1Delta cells, suggesting that in the 
      absence of Coa2 or Shy1, Cox1 forms an unstable conformer. Overexpression of 
      Cox10 or Cox5a and Cox6 or attenuation of the proteolytic activity of the m-AAA 
      protease partially restores respiration in coa2Delta cells. The matrix-localized 
      Coa2 protein may aid in stabilizing an early Cox1 intermediate containing the 
      nuclear subunits Cox5a and Cox6.
FAU - Pierrel, Fabien
AU  - Pierrel F
AD  - University of Utah Health Sciences Center, Department of Medicine, Salt Lake 
      City, UT 84132, USA.
FAU - Khalimonchuk, Oleh
AU  - Khalimonchuk O
FAU - Cobine, Paul A
AU  - Cobine PA
FAU - Bestwick, Megan
AU  - Bestwick M
FAU - Winge, Dennis R
AU  - Winge DR
LA  - eng
GR  - R01 ES003817/ES/NIEHS NIH HHS/United States
GR  - R37 ES003817/ES/NIEHS NIH HHS/United States
GR  - ES 03817/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080609
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Coa2 protein, S cerevisiae)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Protein Subunits)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 1.9.3.1 (Cox1 protein, S cerevisiae)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Electron Transport Complex IV/*biosynthesis/metabolism
MH  - Gene Deletion
MH  - Genetic Complementation Test
MH  - Membrane Proteins/*metabolism
MH  - Mitochondrial Membranes/metabolism
MH  - Mitochondrial Proteins/metabolism
MH  - Protein Biosynthesis
MH  - Protein Processing, Post-Translational
MH  - Protein Subunits/metabolism
MH  - Saccharomyces cerevisiae/cytology/*enzymology/growth & development
MH  - Saccharomyces cerevisiae Proteins/biosynthesis/*metabolism
MH  - Solubility
MH  - Suppression, Genetic
MH  - Thermodynamics
PMC - PMC2519701
EDAT- 2008/06/11 09:00
MHDA- 2008/08/30 09:00
PMCR- 2008/12/01
CRDT- 2008/06/11 09:00
PHST- 2008/06/11 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/06/11 09:00 [entrez]
PHST- 2008/12/01 00:00 [pmc-release]
AID - MCB.00057-08 [pii]
AID - 0057-08 [pii]
AID - 10.1128/MCB.00057-08 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2008 Aug;28(16):4927-39. doi: 10.1128/MCB.00057-08. Epub 2008 Jun 
      9.