PMID- 18547323
OWN - NLM
STAT- MEDLINE
DCOM- 20090202
LR  - 20081031
IS  - 1600-0625 (Electronic)
IS  - 0906-6705 (Linking)
VI  - 17
IP  - 11
DP  - 2008 Nov
TI  - The use of ITS DNA sequence analysis and MALDI-TOF mass spectrometry in 
      diagnosing an infection with Fusarium proliferatum.
PG  - 965-71
LID - 10.1111/j.1600-0625.2008.00726.x [doi]
AB  - Although mycoses are among the most common diseases worldwide, infections with 
      Fusarium spp. occur only rarely. Mostly patients suffering from underlying immune 
      deficiency are infected with this mould, resulting in a considerably decreasing 
      prognosis. In immunocompromised patients, cutaneous manifestations are more often 
      associated with Fusarium sp. than with Candida sp. or Aspergillus sp. We describe 
      one patient with acute lymphoblastic leukaemia, who was first treated with 
      chemotherapy after GMALL protocol 07/03. After relapse, the patient was 
      successfully transplanted in second remission with a human leukocyte antigen 
      (HLA)-matched unrelated peripheral blood stem cell graft. Ten months later, the 
      patient died from respiratory insufficiency and recurrence of leukaemia. 
      Previously, Aspergillus antigen was detected in blood. In the latter course, 
      disseminated papules appeared. One of these was examined histologically and 
      mycologically. Conventional cultural diagnostics led to the diagnosis of a 
      fusariosis, further supported by internal transcribed spacer (ITS) sequencing and 
      matrix assisted laser desorption/ionisation-time-of-flight mass spectrometry 
      (MALDI-TOF) mass spectrometry, both determining the isolated strain as Fusarium 
      proliferatum, which is a very infrequent pathogen within this genus. Our 
      investigations underline the potential of MALDI-TOF MS based identification of 
      Fusarium species as an innovative, time and cost efficient alternative to ITS 
      sequencing.
FAU - Seyfarth, Florian
AU  - Seyfarth F
AD  - Klinik fur Dermatologie und Dermatologische Allergologie, Universitatsklinikum 
      Jena, Jena, Germany. florian.seyfarth@med.uni-jena.de
FAU - Ziemer, Mirjana
AU  - Ziemer M
FAU - Sayer, Herbert G
AU  - Sayer HG
FAU - Burmester, Anke
AU  - Burmester A
FAU - Erhard, Marcel
AU  - Erhard M
FAU - Welker, Martin
AU  - Welker M
FAU - Schliemann, Sibylle
AU  - Schliemann S
FAU - Straube, Eberhard
AU  - Straube E
FAU - Hipler, Uta-Christina
AU  - Hipler UC
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20080610
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (DNA, Ribosomal Spacer)
RN  - 0 (RNA, Ribosomal, 5.8S)
SB  - IM
MH  - Adult
MH  - DNA, Ribosomal Spacer/chemistry/*genetics
MH  - Fatal Outcome
MH  - Female
MH  - Fusarium/classification/genetics/*isolation & purification
MH  - Humans
MH  - Mycoses/*diagnosis/drug therapy/microbiology
MH  - Phylogeny
MH  - RNA, Ribosomal, 5.8S/genetics
MH  - Sequence Analysis, DNA/*methods
MH  - Skin/drug effects/microbiology/pathology
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 2008/06/13 09:00
MHDA- 2009/02/03 09:00
CRDT- 2008/06/13 09:00
PHST- 2008/06/13 09:00 [pubmed]
PHST- 2009/02/03 09:00 [medline]
PHST- 2008/06/13 09:00 [entrez]
AID - EXD726 [pii]
AID - 10.1111/j.1600-0625.2008.00726.x [doi]
PST - ppublish
SO  - Exp Dermatol. 2008 Nov;17(11):965-71. doi: 10.1111/j.1600-0625.2008.00726.x. Epub 
      2008 Jun 10.