PMID- 18548461
OWN - NLM
STAT- MEDLINE
DCOM- 20080905
LR  - 20171116
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 29
IP  - 11
DP  - 2008 Jun
TI  - Separation of alpha-, beta-, gamma-, delta-tocopherols and alpha-tocopherol 
      acetate on a pentaerythritol diacrylate monostearate-ethylene dimethacrylate 
      monolith by capillary electrochromatography.
PG  - 2301-9
LID - 10.1002/elps.200700689 [doi]
AB  - This work reports the first use of a monolith with method development for the 
      separation of tocopherol (TOH) compounds by CEC with UV detection. A 
      pentaerythritol diacrylate monostearate-ethylene dimethacrylate (PEDAS-EDMA) 
      monolithic column has been investigated for an optimised condition to separate 
      alpha-, beta-, gamma- and delta-TOHs, and alpha-tocopherol acetate (TAc). The 
      PEDAS-EDMA monolith showed a remarkably good selectivity for separation of the 
      TOH isomers including the beta- and gamma-isomers which are not easily separated 
      by standard C8 or C18 particle-packed columns. Retention studies indicated that 
      an RP mechanism was involved in the separation on the PEDAS-EDMA column, but 
      polar interactions with the underlying ester and hydroxyl groups enhanced the 
      separation of the problematic beta- and gamma-isomers. Separation of all the 
      compounds was achieved within 25 min using 3:10:87 v/v/v 100 mM Tris buffer (pH 
      9.3)/methanol/ACN as the mobile phase. The method was successfully applied to a 
      pharmaceutical sample with recoveries from 93 to 99%. Intraday and interday 
      precisions (%RSD) for peak area and retention time were less than 2.3. LODs for 
      all four TOHs and TAc were below 1 ppm.
FAU - Chaisuwan, Patcharin
AU  - Chaisuwan P
AD  - Flow Innovation-Research for Science and Technology Laboratories, Department of 
      Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
FAU - Nacapricha, Duangjai
AU  - Nacapricha D
FAU - Wilairat, Prapin
AU  - Wilairat P
FAU - Jiang, Zhengjin
AU  - Jiang Z
FAU - Smith, Norman W
AU  - Smith NW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Acrylates)
RN  - 0 (Methacrylates)
RN  - 0 (Stearates)
RN  - 0 (pentaerythritol diacrylate monostearate)
RN  - 7BK5G69305 (ethylene dimethacrylate)
RN  - 8EF1Z1238F (gamma-Tocopherol)
RN  - 9U6A490501 (beta-Tocopherol)
RN  - H4N855PNZ1 (alpha-Tocopherol)
RN  - JU84X1II0N (delta-tocopherol)
RN  - R0ZB2556P8 (Tocopherols)
SB  - IM
MH  - Acrylates/chemistry
MH  - Capillary Electrochromatography/*methods
MH  - Methacrylates/chemistry
MH  - Stearates/chemistry
MH  - Tocopherols/*isolation & purification
MH  - alpha-Tocopherol/*analogs & derivatives/*isolation & purification
MH  - beta-Tocopherol/*isolation & purification
MH  - gamma-Tocopherol/*isolation & purification
EDAT- 2008/06/13 09:00
MHDA- 2008/09/06 09:00
CRDT- 2008/06/13 09:00
PHST- 2008/06/13 09:00 [pubmed]
PHST- 2008/09/06 09:00 [medline]
PHST- 2008/06/13 09:00 [entrez]
AID - 10.1002/elps.200700689 [doi]
PST - ppublish
SO  - Electrophoresis. 2008 Jun;29(11):2301-9. doi: 10.1002/elps.200700689.