PMID- 18549405
OWN - NLM
STAT- MEDLINE
DCOM- 20081128
LR  - 20080929
IS  - 1349-7006 (Electronic)
IS  - 1347-9032 (Linking)
VI  - 99
IP  - 9
DP  - 2008 Sep
TI  - Diffuse large B-cell lymphoma after transformation from low-grade follicular 
      lymphoma: morphological, immunohistochemical, and FISH analyses.
PG  - 1760-8
LID - 10.1111/j.1349-7006.2008.00873.x [doi]
AB  - Follicular lymphoma (FL) is one of the most common subtypes of non-Hodgkin 
      lymphoma and frequently transforms to diffuse large B-cell lymphoma (DLBCL). To 
      clarify some aspects of the natural history of FL, we retrospectively examined 43 
      consecutive patients who had DLBCL with pre- or coexisting FL grade 1 or 2. The 
      patients comprised 22 men and 21 women with a median age of 53 years. Most of the 
      patients (34/43) showed advanced-stage (III or IV) disease initially. We examined 
      both FL and DLBCL components morphologically, immunohistochemically, and by 
      interface fluorescence in situ hybridization (FISH: IGH/BCL2 fusion, BCL6 
      translocation) analysis. Most of the DLBCLs were classified as the centroblastic 
      subtype, with two exceptions of the anaplastic subtype. Immunohistochemical 
      analysis of both the FL and DLBCL components revealed the following respective 
      positivity rates: CD20 100%/100%, CD10 86%/66%, Bcl-2 96%/91%, Bcl-6 84%/88%, 
      MUM1 16%/34%, CD30 0%/20%, CD138 0%/0%, and CD5 0%/3%. Loss of CD10 (6/36, 17%) 
      and gain of MUM1 (7/28, 25%) and CD30 (5/21, 24%) through transformation were not 
      infrequent. High positivity rates for Bcl-2 and Bcl-6 were maintained throughout 
      transformation. Among the DLBCLs, 84% were classified as the germinal center 
      B-cell phenotype (GCB) and 16% as non-GCB in accordance with the criteria of Hans 
      et al. IGH/BCL2 fusion was detected by FISH in 89% of FLs and 82% of DLBCLs. BCL6 
      translocation was detected in 1/6 (17%) DLBCLs without IGH/BCL2 fusion. Thus, 
      although the morphological features and FISH results for DLBCL were consistent 
      with transformed FL, the immunophenotype showed wide heterogeneity.
FAU - Maeshima, Akiko Miyagi
AU  - Maeshima AM
AD  - Clinical Laboratory, National Cancer Center Hospital, Tsukiji 5-1-1, Chuo-ku, 
      Tokyo 104-0045, Japan. akmaeshi@ncc.go.jp
FAU - Omatsu, Mutsuko
AU  - Omatsu M
FAU - Nomoto, Junko
AU  - Nomoto J
FAU - Maruyama, Dai
AU  - Maruyama D
FAU - Kim, Sung-Won
AU  - Kim SW
FAU - Watanabe, Takashi
AU  - Watanabe T
FAU - Kobayashi, Yukio
AU  - Kobayashi Y
FAU - Tobinai, Kensei
AU  - Tobinai K
FAU - Matsuno, Yoshihiro
AU  - Matsuno Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080628
PL  - England
TA  - Cancer Sci
JT  - Cancer science
JID - 101168776
SB  - IM
MH  - *Cell Transformation, Neoplastic
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Follicular/immunology/*pathology
MH  - Lymphoma, Large B-Cell, Diffuse/immunology/*pathology
MH  - Male
MH  - Middle Aged
EDAT- 2008/06/14 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/06/14 09:00
PHST- 2008/06/14 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/06/14 09:00 [entrez]
AID - CAS873 [pii]
AID - 10.1111/j.1349-7006.2008.00873.x [doi]
PST - ppublish
SO  - Cancer Sci. 2008 Sep;99(9):1760-8. doi: 10.1111/j.1349-7006.2008.00873.x. Epub 
      2008 Jun 28.