PMID- 18549444
OWN - NLM
STAT- MEDLINE
DCOM- 20080915
LR  - 20211020
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 153
IP  - 2
DP  - 2008 Aug
TI  - Induction of IL-10 producing CD4+ T cells with regulatory activities by 
      stimulation with IL-10 gene-modified bone marrow derived dendritic cells.
PG  - 258-68
LID - 10.1111/j.1365-2249.2008.03689.x [doi]
AB  - Dendritic cells (DCs) can induce both tolergenic as well as effective immune 
      responses in the lung. Pulmonary DCs producing interleukin (IL)-10 mediated 
      tolerance induced by respiratory exposure to antigen. IL-10 is an important 
      immunosuppressive cytokine, which inhibits maturation and function of DC. To 
      assess whether IL-10 producing DCs can exert the tolergenic effect through the 
      differentiation of regulatory T cells, bone marrow derived DCs were genetically 
      modified by IL-10 expressing adenovirus. IL-10 gene modified DCs (Ad-IL-10-DC) 
      displayed a characteristic phenotype of immature DCs. Here we showed that in 
      vitro repetitive stimulation of naive DO11.10 CD4(+) T cells with Ad-IL-10-DCs 
      resulted in a development of IL-10 producing T-cell regulatory cells. These T 
      cells could not proliferate well but also lost their ability to produce 
      interferon-gamma upon restimulation with irradiated splenocytes and ovalbumin 
      peptide. Furthermore, in co-culture experiments these T cells inhibited the 
      antigen-driven proliferation of naive CD4+ T cells in a dose-dependent manner. 
      Our findings demonstrated that IL-10 producing DCs had the potential to induce 
      the differentiation of Tr1-like cells and suggested their therapeutic use.
FAU - Fu, C-L
AU  - Fu CL
AD  - Graduate Institute of Immunology, College of Medicine, National Taiwan 
      University, Taiwan, ROC.
FAU - Chuang, Y-H
AU  - Chuang YH
FAU - Huang, H-Y
AU  - Huang HY
FAU - Chiang, B-L
AU  - Chiang BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080628
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Bone Marrow Cells/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cell Differentiation
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Genetic Vectors/genetics/pharmacology
MH  - Immune Tolerance
MH  - Interferon-gamma/immunology
MH  - Interleukin-10/genetics/*immunology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Transgenic
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Transduction, Genetic/methods
PMC - PMC2492900
EDAT- 2008/06/14 09:00
MHDA- 2008/09/16 09:00
PMCR- 2009/08/01
CRDT- 2008/06/14 09:00
PHST- 2008/06/14 09:00 [pubmed]
PHST- 2008/09/16 09:00 [medline]
PHST- 2008/06/14 09:00 [entrez]
PHST- 2009/08/01 00:00 [pmc-release]
AID - CEI3689 [pii]
AID - 10.1111/j.1365-2249.2008.03689.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2008 Aug;153(2):258-68. doi: 10.1111/j.1365-2249.2008.03689.x. 
      Epub 2008 Jun 28.