PMID- 18550142
OWN - NLM
STAT- MEDLINE
DCOM- 20080918
LR  - 20230228
IS  - 0042-6822 (Print)
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 378
IP  - 1
DP  - 2008 Aug 15
TI  - A model for the dynamic nuclear/nucleolar/cytoplasmic trafficking of the porcine 
      reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein based on 
      live cell imaging.
PG  - 34-47
LID - 10.1016/j.virol.2008.04.037 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV), an arterivirus, in 
      common with many other positive strand RNA viruses, encodes a nucleocapsid (N) 
      protein which can localise not only to the cytoplasm but also to the nucleolus in 
      virus-infected cells and cells over-expressing N protein. The dynamic trafficking 
      of positive strand RNA virus nucleocapsid proteins and PRRSV N protein in 
      particular between the cytoplasm and nucleolus is unknown. In this study live 
      imaging of permissive and non-permissive cell lines, in conjunction with 
      photo-bleaching (FRAP and FLIP), was used to investigate the trafficking of 
      fluorescent labeled (EGFP) PRRSV-N protein. The data indicated that EGFP-PRRSV-N 
      protein was not permanently sequestered to the nucleolus and had equivalent 
      mobility to cellular nucleolar proteins. Further the nuclear import of N protein 
      appeared to occur faster than nuclear export, which may account for the observed 
      relative distribution of N protein between the cytoplasm and the nucleolus.
FAU - You, Jae-Hwan
AU  - You JH
AD  - Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds, UK.
FAU - Howell, Gareth
AU  - Howell G
FAU - Pattnaik, Asit K
AU  - Pattnaik AK
FAU - Osorio, Fernando A
AU  - Osorio FA
FAU - Hiscox, Julian A
AU  - Hiscox JA
LA  - eng
GR  - BB/D524875/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080612
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - Animals
MH  - Cell Line
MH  - Cell Nucleolus/*metabolism/ultrastructure
MH  - Cell Nucleus/*metabolism/ultrastructure
MH  - Cytoplasm/*metabolism/ultrastructure
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Microscopy, Confocal
MH  - Nucleocapsid Proteins/genetics/*metabolism
MH  - Photobleaching
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Protein Transport
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Swine
PMC - PMC7103367
EDAT- 2008/06/14 09:00
MHDA- 2008/09/19 09:00
PMCR- 2008/06/12
CRDT- 2008/06/14 09:00
PHST- 2008/02/08 00:00 [received]
PHST- 2008/03/07 00:00 [revised]
PHST- 2008/04/19 00:00 [accepted]
PHST- 2008/06/14 09:00 [pubmed]
PHST- 2008/09/19 09:00 [medline]
PHST- 2008/06/14 09:00 [entrez]
PHST- 2008/06/12 00:00 [pmc-release]
AID - S0042-6822(08)00268-7 [pii]
AID - 10.1016/j.virol.2008.04.037 [doi]
PST - ppublish
SO  - Virology. 2008 Aug 15;378(1):34-47. doi: 10.1016/j.virol.2008.04.037. Epub 2008 
      Jun 12.