PMID- 18550674
OWN - NLM
STAT- MEDLINE
DCOM- 20080902
LR  - 20220331
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 82
IP  - 16
DP  - 2008 Aug
TI  - The high prevalence of herpes simplex virus type 1 DNA in human trigeminal 
      ganglia is not a function of age or gender.
PG  - 8230-4
LID - 10.1128/JVI.00686-08 [doi]
AB  - The purpose of this study was to determine the presence and copy numbers of 
      herpes simplex virus type 1 (HSV-1) DNA in human trigeminal ganglia (TG) with 
      respect to age, gender, and postmortem interval (PMI). Human TG (n = 174, 
      obtained from the Oregon Brain Bank, with data on age, gender, and PMI) were 
      analyzed for HSV-1 DNA copies (HSV-1 DNA polymerase gene) by using real-time PCR. 
      We found that 89.1% (131/147) of subjects and 90.1% (155/174) of TG contained 
      HSV-1 DNA. The copy numbers of HSV-1 DNA in the positives ranged from very high 
      (>10(6)) to very low (5). These data confirm and strengthen our previous findings 
      that subjects were positive for HSV-1 DNA in tears (46/50; 92%) and saliva 
      (47/50; 94%). These TG data and tear and saliva data demonstrated considerable 
      variability in copy numbers of HSV-1 DNA per subject. Statistical analysis showed 
      no significant relationship between gender and copy number, age and copy number, 
      or PMI and copy number for each pair of variables. A factorial analysis of 
      gender, age, and PMI with respect to copy number also showed no statistical 
      significance. This is the first study that provides statistical analysis that 
      documents that the prevalence of HSV-1 DNA in the human TG is not a function of 
      either gender or age.
FAU - Hill, James M
AU  - Hill JM
AD  - Department of Ophthalmology, Louisiana State University Health Sciences Center, 
      New Orleans, Louisiana, USA. jhill@lsuhsc.edu
FAU - Ball, Melvyn J
AU  - Ball MJ
FAU - Neumann, Donna M
AU  - Neumann DM
FAU - Azcuy, Ann M
AU  - Azcuy AM
FAU - Bhattacharjee, Partha S
AU  - Bhattacharjee PS
FAU - Bouhanik, Saadallah
AU  - Bouhanik S
FAU - Clement, Christian
AU  - Clement C
FAU - Lukiw, Walter J
AU  - Lukiw WJ
FAU - Foster, Timothy P
AU  - Foster TP
FAU - Kumar, Manish
AU  - Kumar M
FAU - Kaufman, Herbert E
AU  - Kaufman HE
FAU - Thompson, Hilary W
AU  - Thompson HW
LA  - eng
GR  - R01 EY006311/EY/NEI NIH HHS/United States
GR  - EY002377/EY/NEI NIH HHS/United States
GR  - EY002672/EY/NEI NIH HHS/United States
GR  - F32 EY016316/EY/NEI NIH HHS/United States
GR  - F32EY016316/EY/NEI NIH HHS/United States
GR  - P30 AG008017/AG/NIA NIH HHS/United States
GR  - P30 EY002377/EY/NEI NIH HHS/United States
GR  - AG18031/AG/NIA NIH HHS/United States
GR  - AG02085/AG/NIA NIH HHS/United States
GR  - R01 AG018031/AG/NIA NIH HHS/United States
GR  - R01 EY002672/EY/NEI NIH HHS/United States
GR  - EY006311/EY/NEI NIH HHS/United States
GR  - AG08017/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080611
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Age Factors
MH  - Aged
MH  - DNA, Viral/*metabolism
MH  - Female
MH  - Herpesvirus 1, Human/*metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Regression Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Saliva/virology
MH  - Sex Factors
MH  - Tears/virology
MH  - Trigeminal Ganglion/*virology
MH  - Virus Latency
PMC - PMC2519549
EDAT- 2008/06/14 09:00
MHDA- 2008/09/03 09:00
PMCR- 2008/12/01
CRDT- 2008/06/14 09:00
PHST- 2008/06/14 09:00 [pubmed]
PHST- 2008/09/03 09:00 [medline]
PHST- 2008/06/14 09:00 [entrez]
PHST- 2008/12/01 00:00 [pmc-release]
AID - JVI.00686-08 [pii]
AID - 0686-08 [pii]
AID - 10.1128/JVI.00686-08 [doi]
PST - ppublish
SO  - J Virol. 2008 Aug;82(16):8230-4. doi: 10.1128/JVI.00686-08. Epub 2008 Jun 11.