PMID- 18551408
OWN - NLM
STAT- MEDLINE
DCOM- 20081006
LR  - 20191110
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 7
IP  - 2
DP  - 2008 May 7
TI  - Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem 
      cell transplantation: the experience of the Bone Marrow Transplantation Unit of 
      FUNFARME/BRAZIL using FISH.
PG  - 417-23
AB  - Investigation of the efficacy of allogeneic hematopoietic stem cell 
      transplantation (allo-HSCT) in chronic myeloid leukemia patients is essential to 
      predict prognosis and survival. In 20 patients treated at the Bone Marrow 
      Transplantation Unit of Sao Jose do Rio Preto (Sao Paulo, Brazil), we used 
      fluorescence in situ hybridization (FISH) to investigate the frequency of cells 
      with BCR/ABL rearrangement at diagnosis and at distinct intervals after allo-HSCT 
      until complete cytogenetic remission (CCR). We investigated the disease-free 
      survival, overall survival in 3 years and transplant-related mortality rates, 
      too. Bone marrow samples were collected at 1, 2, 3, 4, 6, 12, and 24 months after 
      transplantation and additional intervals as necessary. Success rate of the FISH 
      analyses was 100%. CCR was achieved in 75% of the patients, within on average of 
      3.9 months; 45% patients showed CCR within 60 days after HSCT. After 3 years of 
      the allo-HSCT, overall survival rate was 60%, disease-free survival was 50% and 
      the transplant-related mortality rate was 40%. The study demonstrated that the 
      BCR-ABL FISH assay is useful for follow-up of chronic myeloid leukemia patients 
      after HSCT and that the clinical outcome parameters in our patient cohort were 
      similar to those described for other bone marrow transplantation units.
FAU - Vendrame-Goloni, C B
AU  - Vendrame-Goloni CB
AD  - Departamento de Biologia, Instituto de Biociencias Letras e Ciencias Exatas, 
      Universidade Estadual Paulista, Sao Jose do Rio Preto, SP, Brasil. 
      cristina.benitez@ig.com.br
FAU - Carvalho-Salles, A B
AU  - Carvalho-Salles AB
FAU - Ruiz, M A
AU  - Ruiz MA
FAU - Ricci Junior, O
AU  - Ricci Junior O
FAU - Varella-Garcia, M
AU  - Varella-Garcia M
FAU - Fett-Conte, A C
AU  - Fett-Conte AC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080507
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Marrow Transplantation
MH  - Brazil
MH  - Disease-Free Survival
MH  - Hematopoietic Stem Cell Transplantation/*methods
MH  - Hospital Departments
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/mortality/*surgery
MH  - Middle Aged
MH  - Prognosis
MH  - Survival Rate
MH  - Transplantation, Homologous
MH  - Treatment Outcome
EDAT- 2008/06/14 09:00
MHDA- 2008/10/07 09:00
CRDT- 2008/06/14 09:00
PHST- 2008/06/14 09:00 [pubmed]
PHST- 2008/10/07 09:00 [medline]
PHST- 2008/06/14 09:00 [entrez]
AID - gmr396 [pii]
AID - 10.4238/vol7-2gmr396 [doi]
PST - epublish
SO  - Genet Mol Res. 2008 May 7;7(2):417-23. doi: 10.4238/vol7-2gmr396.