PMID- 18551526
OWN - NLM
STAT- MEDLINE
DCOM- 20080804
LR  - 20181201
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 100
IP  - 5
DP  - 2008 Aug 1
TI  - Characterization of porous poly(D,L-lactic-co-glycolic acid) sponges fabricated 
      by supercritical CO2 gas-foaming method as a scaffold for three-dimensional 
      growth of Hep3B cells.
PG  - 998-1009
LID - 10.1002/bit.21824 [doi]
AB  - This study presents the application of the porous poly(D,L-lactic-co-glycolic 
      acid) (PLGA) sponges fabricated from an organic solvent free supercritical gas 
      foaming technique. Two formulations of PLGA sponges with different co-polymer 
      compositions (85:15 and 50:50) were fabricated as novel scaffolds to guide human 
      hepatoma cell line, Hep3B cell growth in vitro. The PLGA sponges showed desirable 
      biodegradability and exhibited uniform pore size distribution with moderate 
      interconnectivity. It was observed in this study that cells cultured on PLGA 
      sponges showed lower proliferation rate as compared to the control during 14 days 
      of culture as measured by using total DNA and methylthiazol tetrazolium (MTT) 
      assays. However, the cells cultured on the sponges tended to aggregate to form 
      cell islets which were able to express better hepatic functions. The 
      enzyme-linked immunosorbent assay (ELISA) results showed that the cell-sponge 
      constructs secreted 1.5-3.0 times more albumin than the control when normalized 
      to cellular content. In a similar fashion, its detoxification ability was also 
      predominantly higher than that of the control as indicated by the 
      ethoxyresorufin-O-deethylase (EROD) results. By comparing the cells growing on 
      the two formulations of PLGA sponges, it was found that the PLGA 85:15 sponge 
      exhibited better conductive and desirable environment for hep3B cells as 
      justified by better cell infiltration, higher proliferation and hepatic function 
      than the PLGA 50:50 sponge.
CI  - (c) 2008 Wiley Periodicals, Inc.
FAU - Zhu, Xin Hao
AU  - Zhu XH
AD  - Department of Chemical & Biomolecular Engineering, National University of 
      Singapore, 4 Engineering Drive 4, Singapore, Singapore.
FAU - Lee, Lai Yeng
AU  - Lee LY
FAU - Jackson, Jie Sheng Hong
AU  - Jackson JS
FAU - Tong, Yen Wah
AU  - Tong YW
FAU - Wang, Chi-Hwa
AU  - Wang CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Biocompatible Materials)
RN  - 0 (Gases)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Biocompatible Materials/*chemistry
MH  - Carbon Dioxide/chemistry
MH  - Cell Culture Techniques/*methods
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Chromatography, Supercritical Fluid/methods
MH  - Gases
MH  - Hepatocytes/*cytology/*physiology
MH  - Humans
MH  - Lactic Acid/*chemistry
MH  - Materials Testing
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Porosity
MH  - Tissue Engineering/*methods
EDAT- 2008/06/14 09:00
MHDA- 2008/08/05 09:00
CRDT- 2008/06/14 09:00
PHST- 2008/06/14 09:00 [pubmed]
PHST- 2008/08/05 09:00 [medline]
PHST- 2008/06/14 09:00 [entrez]
AID - 10.1002/bit.21824 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2008 Aug 1;100(5):998-1009. doi: 10.1002/bit.21824.