PMID- 18552200
OWN - NLM
STAT- MEDLINE
DCOM- 20081210
LR  - 20220317
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 20
IP  - 6
DP  - 2008 Jun
TI  - Arabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 forms a nuclear E3 ubiquitin ligase with 
      DDB1 and CUL4 that is involved in multiple plant developmental processes.
PG  - 1437-55
LID - 10.1105/tpc.108.058891 [doi]
AB  - The human DDB1-CUL4 ASSOCIATED FACTOR (DCAF) proteins have been reported to 
      interact directly with UV-DAMAGED DNA BINDING PROTEIN1 (DDB1) through the WDxR 
      motif in their WD40 domain and function as substrate-recognition receptors for 
      CULLIN4-based E3 ubiquitin ligases. Here, we identified and characterized a 
      homolog of human DCAF1/VprBP in Arabidopsis thaliana. Yeast two-hybrid analysis 
      demonstrated the physical interaction between DCAF1 and DDB1 from Arabidopsis, 
      which is likely mediated via the WD40 domain of DCAF1 that contains two WDxR 
      motifs. Moreover, coimmunoprecipitation assays showed that DCAF1 associates with 
      DDB1, RELATED TO UBIQUITIN-modified CUL4, and the COP9 signalosome in vivo but 
      not with CULLIN-ASSOCIATED and NEDDYLATION-DISSOCIATED1, CONSTITUTIVE 
      PHOTOMORPHOGENIC1 (COP1), or the COP10-DET1-DDB1 complex, supporting the 
      existence of a distinct Arabidopsis CUL4 E3 ubiquitin ligase, the CUL4-DDB1-DCAF1 
      complex. Transient expression of fluorescently tagged DCAF1, DDB1, and CUL4 in 
      onion epidermal cells showed their colocalization in the nucleus, consistent with 
      the notion that the CUL4-DDB1-DCAF1 complex functions as a nuclear E3 ubiquitin 
      ligase. Genetic and phenotypic analysis of two T-DNA insertion mutants of DCAF1 
      showed that embryonic development of the dcaf1 homozygote is arrested at the 
      globular stage, indicating that DCAF1 is essential for plant embryogenesis. 
      Reducing the levels of DCAF1 leads to diverse developmental defects, implying 
      that DCAF1 might be involved in multiple developmental pathways.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Peking-Yale Joint Center of Plant Molecular Genetics and Agrobiotechnology, 
      College of Life Sciences, Peking University, Beijing 100871, China.
FAU - Feng, Suhua
AU  - Feng S
FAU - Chen, Fangfang
AU  - Chen F
FAU - Chen, Haodong
AU  - Chen H
FAU - Wang, Jia
AU  - Wang J
FAU - McCall, Chad
AU  - McCall C
FAU - Xiong, Yue
AU  - Xiong Y
FAU - Deng, Xing Wang
AU  - Deng XW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080613
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (CULLIN4 protein, Arabidopsis)
RN  - 0 (Cullin Proteins)
RN  - 0 (DET1 protein, Arabidopsis)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (T-DNA)
RN  - EC 2.3.2.23 (COP10 protein, Arabidopsis)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Arabidopsis/embryology/genetics/*metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism/physiology
MH  - Cullin Proteins/genetics/*metabolism/physiology
MH  - DNA, Bacterial/genetics
MH  - DNA-Binding Proteins/genetics/metabolism/physiology
MH  - Gene Expression Regulation, Developmental
MH  - Gene Expression Regulation, Plant
MH  - Humans
MH  - Immunoprecipitation
MH  - Intracellular Signaling Peptides and Proteins
MH  - Molecular Sequence Data
MH  - Mutagenesis, Insertional
MH  - Nuclear Proteins/genetics/metabolism/physiology
MH  - Onions/genetics/metabolism
MH  - Plants, Genetically Modified/embryology/genetics/metabolism
MH  - Protein Binding
MH  - Sequence Homology, Amino Acid
MH  - Ubiquitin-Conjugating Enzymes/genetics/metabolism/physiology
MH  - Ubiquitin-Protein Ligases
PMC - PMC2483375
EDAT- 2008/06/17 09:00
MHDA- 2008/12/17 09:00
PMCR- 2009/06/01
CRDT- 2008/06/17 09:00
PHST- 2008/06/17 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/06/17 09:00 [entrez]
PHST- 2009/06/01 00:00 [pmc-release]
AID - tpc.108.058891 [pii]
AID - 058891 [pii]
AID - 10.1105/tpc.108.058891 [doi]
PST - ppublish
SO  - Plant Cell. 2008 Jun;20(6):1437-55. doi: 10.1105/tpc.108.058891. Epub 2008 Jun 
      13.