PMID- 18553169
OWN - NLM
STAT- MEDLINE
DCOM- 20090714
LR  - 20170506
IS  - 1387-2176 (Print)
IS  - 1387-2176 (Linking)
VI  - 10
IP  - 6
DP  - 2008 Dec
TI  - Fabrication and testing of a PDMS multi-stacked hand-operated LOC for use in 
      portable immunosensing systems.
PG  - 859-868
LID - 10.1007/s10544-008-9200-9 [doi]
AB  - This paper presents the development of a reliable multi-liquid lab-on-a-chip 
      (LOC), with a hand-operated mechanism, for the application in portable 
      immunosensing systems. To control the transport of multiple liquids without any 
      external equipment, we utilize capillary attraction force for filling and surface 
      tension for stopping liquid flow. As a driving force, hydraulic pressure caused 
      by the elastic deformation of a liquid reservoir transfers liquid stopped at 
      passive valves. The proposed LOC successfully demonstrates a reliable sequential 
      liquid transfer within the reaction channel. To highlight its feasibility as a 
      portable diagnostic system, we performed the electrochemical immunoassay 
      measuring antibody concentrations within the fabricated LOC. As a test 
      biorecognition reaction, the anti-dinitrophenyl (DNP) antibody with an enzymatic 
      catalysis was selected as the target analyte. The amplified signals obtained from 
      this experiment indicated a high selectivity of the immunosensing LOC.
FAU - Park, Sin Wook
AU  - Park SW
AD  - Division of Electrical & Computer Engineering, Ajou University, Suwon, South 
      Korea.
FAU - Lee, Jun Hwang
AU  - Lee JH
AD  - Department of Molecular Science & Technology, Ajou University, Suwon, South 
      Korea.
FAU - Yoon, Hyun C
AU  - Yoon HC
AD  - Department of Molecular Science & Technology, Ajou University, Suwon, South 
      Korea.
FAU - Kim, Byung Woo
AU  - Kim BW
AD  - Department of Chemical Engineering, Sungkyunkwan University, Suwon, South Korea.
FAU - Sim, Sang Jun
AU  - Sim SJ
AD  - Department of Chemical Engineering, Sungkyunkwan University, Suwon, South Korea.
FAU - Chae, Heeyeop
AU  - Chae H
AD  - Department of Chemical Engineering, Sungkyunkwan University, Suwon, South Korea.
FAU - Yang, Sang Sik
AU  - Yang SS
AD  - Division of Electrical & Computer Engineering, Ajou University, Suwon, South 
      Korea. ssyang@ajou.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biomed Microdevices
JT  - Biomedical microdevices
JID - 100887374
RN  - 0 (Antibodies)
RN  - 0 (Dinitrobenzenes)
SB  - IM
MH  - Animals
MH  - Antibodies/*chemistry
MH  - Dinitrobenzenes/*analysis
MH  - Immunoenzyme Techniques/*instrumentation/*methods
MH  - Microchip Analytical Procedures/*methods
MH  - Sensitivity and Specificity
EDAT- 2008/06/17 09:00
MHDA- 2009/07/15 09:00
CRDT- 2008/06/17 09:00
PHST- 2008/06/17 09:00 [pubmed]
PHST- 2009/07/15 09:00 [medline]
PHST- 2008/06/17 09:00 [entrez]
AID - 10.1007/s10544-008-9200-9 [pii]
AID - 10.1007/s10544-008-9200-9 [doi]
PST - ppublish
SO  - Biomed Microdevices. 2008 Dec;10(6):859-868. doi: 10.1007/s10544-008-9200-9.